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Literature summary for 3.5.1.52 extracted from

  • Joshi, S.; Katiyar, S.; Lennarz, W.J.
    Misfolding of glycoproteins is a prerequisite for peptide; N-glycanase mediated deglycosylation (2005), FEBS Lett., 579, 823-826.
    View publication on PubMed
Search for more literature for 3.5.1.52

Cloned(Commentary)

Cloned (Comment) Organism
gene png1, expression of wild-type and mutant Png1 in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C191A site-directed mutagenesis, inactive mutant Saccharomyces cerevisiae
D235A site-directed mutagenesis, inactive mutant Saccharomyces cerevisiae
H218A site-directed mutagenesis, inactive mutant Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant Png1 from Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc-Asn-Asp-Glu-Ser-Ser + H2O = Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc + Asn-Asp-Glu-Ser-Ser the catalytic triad is formed by Cys191, His218, and Asp235 Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the deglycosylating enzyme catalyzes the hydrolysis of the beta-aspartylglycosylamine bond of asparagine-linked glycopeptides and glycoproteins, the misfolding or denaturation of glycoproteins is a prerequisite for enzyme activity Saccharomyces cerevisiae ?
-
 ?
ovalbumin + H2O deglycosylation of the heat-denatured protein Saccharomyces cerevisiae ?
-
 ?
yeast carboxypeptidase + H2O deglycosylation of heat-denatured protein Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
peptide: N-glycanase
-
 Saccharomyces cerevisiae
Png1p
-
 Saccharomyces cerevisiae
PNGase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Saccharomyces cerevisiae