Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | mouse organ-derived PNGase requires at least 1 mM DTT for maximal activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Mus musculus | |
Fe3+ | - |
Mus musculus | |
Glycoasparagine | - |
Mus musculus | |
Monoiodoacetic acid | 2 mM, significant inhibition by thiol-modification | Mus musculus | |
additional information | no effect: free peptides formed during reaction, mannose, alpha-methyl-D-mannoside, 5 mM Na2SO4 | Mus musculus | |
N-ethylmaleimide | 2 mM, significant inhibition by thiol-modification, alkylation | Mus musculus | |
Zn2+ | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
spleen | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bovine fetuin glycopeptide + H2O | Leu-Asn(Man3Gal3GlcNAc5)-Asp-Ser-Arg | Mus musculus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
liver PNGase | Mus musculus |
6.8 | - |
spleen PNGase | Mus musculus |
7 | - |
L-929 PNGase, brain PNGase, kidney PNGase | Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | no activity below 5.5 | Mus musculus |