Any feedback?
Literature summary for 3.5.1.52 extracted from
- Does an animal peptide N:glycanase have the dual role as an enzyme and a carbohydrate binding protein? (1994), Glycoconj. J., 11, 469-476.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| liberated glycan reaction product | - |
Mus musculus | |
| additional information | no effect: yeast mannan, trimannose | Elizabethkingia meningoseptica | |
| additional information | no effect: free peptides formed during reaction, mannose, alpha-methyl-D-mannoside, 5 mM Na2SO4 | Mus musculus | |
| additional information | no effect: thiol inhibitors, iodoacetamide, N-ethylmaleimide, actinomycete protease inhibitors, leupeptins, chymostatin, pepstatin, Mg2+, Ca2+, Mn2+, EDTA, L-cysteine, phenylmethylsulfonyl fluoride, gamma-D-gluconolactone, yeast mannan, triomannose | Prunus amygdalus var. dulcis | |
| triomannose | - |
Mus musculus |  |
| yeast mannan | - |
Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.525 | - |
Fetuin glycopeptide | - |
Elizabethkingia meningoseptica | |
| 1.46 | - |
Fetuin glycopeptide | - |
Prunus amygdalus var. dulcis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66800 | - |
gel filtration | Prunus amygdalus var. dulcis |
| 105000 | - |
2 * 105000, homodimer, SDS-PAGE, 41% content of hydrophobic acids | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Elizabethkingia meningoseptica | - |
- |
- |
| Mus musculus | - |
- |
- |
| Oryzias latipes | - |
medaka fish | - |
| Prunus amygdalus var. dulcis | - |
almond | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bovine fetuin glycopeptide + H2O | - |
Mus musculus | ? | - |
? | |
| bovine fetuin glycopeptide + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
| fetuin glycopeptide + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
| fetuin glycopeptide + H2O | - |
Prunus amygdalus var. dulcis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Prunus amygdalus var. dulcis |
| dimer | 2 * 105000, homodimer, SDS-PAGE, 41% content of hydrophobic acids | Mus musculus |
| monomer | - |
Elizabethkingia meningoseptica |
| monomer | - |
Prunus amygdalus var. dulcis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
L-929 PNGase, brain PNGase, kidney PNGase | Mus musculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
