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Literature summary for 3.5.1.49 extracted from
- The antigenic and catalytically active formamidase of Paracoccidioides brasiliensis: protein characterization, cDNA and gene cloning, heterologous expression and functional analysis of the recombinant protein (2005), Microbes Infect., 7, 66-77.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the SalI/NotI sites of the Vector pGEX-4T-3, fused to glutathione S-transferase (GST) | Paracoccidioides brasiliensis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | putative, theoretical computational analysis | Paracoccidioides brasiliensis | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
SDS-PAGE and Western blot analysis | Paracoccidioides brasiliensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formamide + H2O | Paracoccidioides brasiliensis | - |
formate + NH3 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccidioides brasiliensis | Q8J0S3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expressed in the soluble form by Escherichia coli XL1 Blue MRF cells (5mg fusion protein per liter culture), purified on a Glutathione Sepharose 4B Resin column, cleaved with thrombin | Paracoccidioides brasiliensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Paracoccidioides brasiliensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.24 | - |
yeast | Paracoccidioides brasiliensis |
| 1.79 | - |
mycelium | Paracoccidioides brasiliensis |
| 2.73 | - |
purified recombinant formamidase | Paracoccidioides brasiliensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formamide + H2O | - |
Paracoccidioides brasiliensis | formate + NH3 | - |
? | |
| formamide + H2O | activity assay | Paracoccidioides brasiliensis | formate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fmd | - |
Paracoccidioides brasiliensis |
| formamidase | - |
Paracoccidioides brasiliensis |
| Formamide amidohydrolase | - |
Paracoccidioides brasiliensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Paracoccidioides brasiliensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
activity assay | Paracoccidioides brasiliensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Paracoccidioides brasiliensis | isoelectric focusing | - |
6 |
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