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Literature summary for 3.5.1.41 extracted from

  • Zhong, X.W.; Wang, X.H.; Tan, X.; Xia, Q.Y.; Xiang, Z.H.; Zhao, P.
    Identification and molecular characterization of a chitin deacetylase from Bombyx mori peritrophic membrane (2014), Int. J. Mol. Sci., 15, 1946-1961.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.41

Cloned(Commentary)

Cloned (Comment) Organism
gene BmCDA7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression in Pichia pastoris strain GS115, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) Bombyx mori

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000, recombinant His-tagged enzyme, SDS-PAGE, x * 41400, about, full-length enzyme, sequence calculation, x * 41260, enzyme without signal peptide, sequence calculation Bombyx mori
41260
-
 x * 41000, recombinant His-tagged enzyme, SDS-PAGE, x * 41400, about, full-length enzyme, sequence calculation, x * 41260, enzyme without signal peptide, sequence calculation Bombyx mori
41400
-
 x * 41000, recombinant His-tagged enzyme, SDS-PAGE, x * 41400, about, full-length enzyme, sequence calculation, x * 41260, enzyme without signal peptide, sequence calculation Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bombyx mori the enzyme catalyses the hydrolysis of the acetamido group in the N-acetylglucosamine units of chitin and chitosan, generating glucosamine units and acetic acid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori H9JW44 gene BmCDA7
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme contains a putative N-glycosylation site at Asn168, and putative O-glycosylation sites at Thr209 and Thr215. The BmCDA7 protein might be both N- and O-glycosylated Bombyx mori

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
epithelium
-
 Bombyx mori
-
larva the BmCDA7 gene is expressed by the newly hatched silkworm larvae until day seven of the fifth instar and is expressed at a high level in the newly exuviated larvae of different instars Bombyx mori
-
midgut
-
 Bombyx mori
-
additional information enzyme expression analysis by reverse transcription-PCR, temporal expression profiles and tissue distribution, overview Bombyx mori
-
peritrophic membrane an acellular structure secreted by midgut cells lines the digestive tract in most insects that has important roles in facilitating food digestion and providing protection to the gut epithelium Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyses the hydrolysis of the acetamido group in the N-acetylglucosamine units of chitin and chitosan, generating glucosamine units and acetic acid Bombyx mori ?
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, recombinant His-tagged enzyme, SDS-PAGE, x * 41400, about, full-length enzyme, sequence calculation, x * 41260, enzyme without signal peptide, sequence calculation Bombyx mori

Synonyms

Synonyms Comment Organism
BmCDA7
-
 Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bombyx mori

pI Value

Organism Comment pI Value Maximum pI Value
Bombyx mori sequence calculation
-
 5.2

General Information

General Information Comment Organism
additional information the enzyme sequence has a 16 amino acid residue signal peptide, a putative polysaccharide deacetylase-like domain (residues 46-182), and 15 cysteine residues present in three clusters, residues 24-83, 183-243, and 332-365, structure comparison, overview Bombyx mori