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Literature summary for 3.5.1.41 extracted from
- A highly N-glycosylated chitin deacetylase derived from a novel strain of Mortierella sp. DY-52 (2011), Biosci. Biotechnol. Biochem., 75, 960-965.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 77.9% residual activity at 10 mM | Mortierella sp. |  |
| Ca2+ | 86% residual activity at 10 mM | Mortierella sp. | |
| Cd2+ | 33.6% residual activity at 10 mM | Mortierella sp. | |
| Co2+ | 79.7% residual activity at 10 mM | Mortierella sp. | |
| Cu2+ | 15.4% residual activity at 10 mM | Mortierella sp. | |
| EDTA | 59.8% residual activity at 10 mM | Mortierella sp. | |
| Fe2+ | 55.6% residual activity at 10 mM | Mortierella sp. | |
| Mn2+ | 68.2% residual activity at 10 mM | Mortierella sp. | |
| Ni2+ | 51.2% residual activity at 10 mM | Mortierella sp. | |
| Pb2+ | 32.3% residual activity at 10 mM | Mortierella sp. | |
| Zn2+ | 74.7% residual activity at 10 mM | Mortierella sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
hexa-N-acetylchitohexaose | at pH 6.0 and 50°C | Mortierella sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Mortierella sp. | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
1 * 52000, SDS-PAGE | Mortierella sp. |
| 67000 | - |
gel filtration | Mortierella sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mortierella sp. | - |
- |
- |
| Mortierella sp. DY-52 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | a highly N-glycosylated protein | Mortierella sp. |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, phenyl Sepharose column chromatography, Sephacryl S-300 gel filtration, and Resource Q column chromatography | Mortierella sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3 | - |
culture filtrate, using water-soluble chitin with degree of deacetylation of 50% as substrate, at pH 6.0 and 50°C | Mortierella sp. |
| 175 | - |
after 58fold purification, using water-soluble chitin with degree of deacetylation of 50% as substrate, at pH 6.0 and 50°C | Mortierella sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| crab crystalline chitin + H2O | worst substrate (0.5% deacetylation) | Mortierella sp. | ? | - |
? | |
| crab swollen chitin + H2O | 1.7% deacetylation | Mortierella sp. | ? | - |
? | |
| di-N-acetylchitobiose + H2O | 0.6% deacetylation | Mortierella sp. | ? | - |
? | |
| di-N-acetylchitobiose + H2O | 0.6% deacetylation | Mortierella sp. DY-52 | ? | - |
? | |
| ethylene glycol chitin + H2O | best substrate (24.5% deacetlyation) | Mortierella sp. | chitosan + acetate | - |
? | |
| hexa-N-acetylchitohexaose + H2O | 33.2% deacetylation | Mortierella sp. | ? | - |
? | |
| hexa-N-acetylchitohexaose + H2O | 33.2% deacetylation | Mortierella sp. DY-52 | ? | - |
? | |
| additional information | no activity with GlcNAc | Mortierella sp. | ? | - |
? | |
| additional information | no activity with GlcNAc | Mortierella sp. DY-52 | ? | - |
? | |
| penta-N-acetylchitopentaose + H2O | 25.8% deacetylation | Mortierella sp. | ? | - |
? | |
| penta-N-acetylchitopentaose + H2O | 25.8% deacetylation | Mortierella sp. DY-52 | ? | - |
? | |
| shrimp alpha-chitin powder + H2O | 0.7% deacetylation | Mortierella sp. | ? | - |
? | |
| shrimp swollen chitin + H2O | 2.1% deacetylation | Mortierella sp. | ? | - |
? | |
| squid beta-chitin powder + H2O | 3.6% deacetylation | Mortierella sp. | ? | - |
? | |
| tetra-N-acetylchitotetraose + H2O | 25.5% deacetylation | Mortierella sp. | ? | - |
? | |
| tetra-N-acetylchitotetraose + H2O | 25.5% deacetylation | Mortierella sp. DY-52 | ? | - |
? | |
| tri-N-acetylchitotriose + H2O | 6.3% deacetylation | Mortierella sp. | ? | - |
? | |
| WSCT-50 + H2O | water-soluble chitin with degree of deacetylation of 50% | Mortierella sp. | ? + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52000, SDS-PAGE | Mortierella sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDA | - |
Mortierella sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Mortierella sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | about 50% activity at 40 and 70°C | Mortierella sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | the enzyme is stable up to 40°C, then rapidly losing activity with less 70% residual activity at 50°C and less than 10% activity at 60°C after 2 h incubation | Mortierella sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Mortierella sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | the enzyme maintains 90% of its activity at pH between 5.0 and 7.0 and about 35% activity at pH 3.0 and 8.0 | Mortierella sp. |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mortierella sp. | isoelectric focusing | 4.8 | 4.2 |
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Release 2023.1 (January 2023)
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