Cloned (Comment) | Organism |
---|---|
three chitin deacetylase cDNAs (RC, D2, and I3/2) are isolated from the cDNA library and sequenced. These cDNAs exhibit features characteristic of chitin deacetylase sequences: the presence of a polysaccharide deacetylase domain, a metal-binding triad, the conserved catalytic residues, and high homology with various chitin deacetylase genes. The cDNAs are cloned in a Pichia pastoris expression system and produced as polyhistidine-tagged proteins. Only recombinant enzyme chitin deacetylase RC is active under the tested conditions | Rhizopus circinans |
General Stability | Organism |
---|---|
good stability in the presence of acetate. Addition of 10 mM or 50 mM acetate to the assay mixture results, respectively, in a 4% or 49% loss of enzyme activity | Rhizopus circinans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 10 mM, 41% inhibition | Rhizopus circinans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM, slightly enhances activity | Rhizopus circinans | |
Mn2+ | 1 mM, slightly enhances activity | Rhizopus circinans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
x * 75000, SDS-PAGE | Rhizopus circinans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Rhizopus circinans | catalyzes hydrolysis of the acetamido groups of N-acetylglucosamine of chitin in fungal cell walls | chitosan + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus circinans | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-deglycosylation results in total loss of the enzyme activity | Rhizopus circinans |
Purification (Comment) | Organism |
---|---|
- |
Rhizopus circinans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
965.1 | - |
- |
Rhizopus circinans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | catalyzes hydrolysis of the acetamido groups of N-acetylglucosamine of chitin in fungal cell walls | Rhizopus circinans | chitosan + acetate | - |
? | |
chitin + H2O | enzyme exhibits high activity on fungal chitosan (78%), shrimp chitosan (83%), partially deacetylated chitin (77%), and chitohexaose (120%) | Rhizopus circinans | ? | - |
? | |
glycol chitin + H2O | - |
Rhizopus circinans | chitosan + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, SDS-PAGE | Rhizopus circinans |
Synonyms | Comment | Organism |
---|---|---|
chitin deacetylase RC | - |
Rhizopus circinans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rhizopus circinans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Rhizopus circinans |