Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 10 mM, 63% inhibition | Mortierella sp. | |
Cu2+ | 10 mM, 58% inhibition | Mortierella sp. | |
EDTA | 1 mM, 45% inhibition. 10 mM, complete inhibition | Mortierella sp. | |
Fe2+ | 10 mM, 65% inhibition | Mortierella sp. | |
Hg2+ | 1 mM, complete inhibition | Mortierella sp. | |
Mg2+ | 1 mM, 43% inhibition | Mortierella sp. | |
Mn2+ | 1 mM, 84% inhibition | Mortierella sp. | |
Ni2+ | 10 mM, 51% inhibition | Mortierella sp. | |
Pb2+ | 1 mM, 88% inhibition | Mortierella sp. | |
Sn2+ | 1 mM, 66% inhibition | Mortierella sp. | |
Zn2+ | 2 mM, 78% inhibition. 10 mM, complete inhibition | Mortierella sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Mortierella sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 1.26fold activation | Mortierella sp. | |
Co2+ | 1 mM, 1.46fold activation | Mortierella sp. | |
Ni2+ | 1 mM, 1.1fold activation | Mortierella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mortierella sp. | - |
- |
- |
Mortierella sp. DY-52 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the highest enzyme activity is observed at 3 days of cultivation, where glucose is added. Powder chitin and swollen chitin also induced the extracellular CDA but in a lesser extent than glucose. Little CDA activity is detected in MeOH addition. The mixtures of glucose plus maltose and glucose plus swollen chitin (1% each) also induce CDA, but the addition of glucose plus swollen chitin delays the maximum induction of enzyme to the 5th day. Enzyme production is maximal at initial pH 4.5-5.0 and decreases dramatically below pH 4.0. About 60% of the maximum CDA production can be achieved at pH 6.0. The optimum temperature is 25-28°C, and less than 60% of the maximum activity was observed at 20°C and 30°C. The optimum agitation speed for enzyme production is 150 rpm | Mortierella sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | the enzyme is active on WSCT-50, glycol chitin, and crab chitosan (DD 71-88%) for deacetylation with a wide range of affinities (7-100%). Among them, WSCT-50 is the most accessible substrate | Mortierella sp. | chitosan + acetate | - |
? | |
chitin + H2O | the enzyme is active on WSCT-50, glycol chitin, and crab chitosan (DD 71-88%) for deacetylation with a wide range of affinities (7-100%). Among them, WSCT-50 is the most accessible substrate | Mortierella sp. DY-52 | chitosan + acetate | - |
? | |
N-acetylglucosamine oligomer + H2O | the enzyme can deacetylate (GlcNAc)2-7. The highest enzyme activity was achieved when chitin heptamer is used as a substrate | Mortierella sp. | ? | - |
? | |
N-acetylglucosamine oligomer + H2O | the enzyme can deacetylate (GlcNAc)2-7. The highest enzyme activity was achieved when chitin heptamer is used as a substrate | Mortierella sp. DY-52 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Mortierella sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 20°C: about 30% of maximal activity, 70°C: about 50% of maximal activity | Mortierella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 40 | 1 h, stable | Mortierella sp. |
50 | - |
1 h, about 30% loss of activity | Mortierella sp. |
60 | - |
1 h, about 35% loss of activity | Mortierella sp. |
70 | - |
1 h, about 85% loss of activity | Mortierella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Mortierella sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | pH 4.5: about 20% of maximal activity, pH 8.5: about 40% of maximal activity | Mortierella sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | 4°C, stable overnight | Mortierella sp. |