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Literature summary for 3.5.1.41 extracted from
- Expression of chitin deacetylase from Colletotrichum lindemuthianum in Pichia pastoris: purification and characterization (2004), Protein Expr. Purif., 38, 196-204.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme might be useful for large scale production of chitosan from chitin | Colletotrichum lindemuthianum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged enzyme in Pichia pastoris strain GS115, secretion of the recombinant enzyme to the culture supernatant | Colletotrichum lindemuthianum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibitory at higher concentration | Colletotrichum lindemuthianum |  |
| Cu2+ | highly inhibitory at higher concentration | Colletotrichum lindemuthianum | |
| Fe2+ | highly inhibitory at higher concentration | Colletotrichum lindemuthianum | |
| Mg2+ | inhibitory at higher concentration | Colletotrichum lindemuthianum | |
| Ni2+ | potent inhibition | Colletotrichum lindemuthianum | |
| Zn2+ | potent inhibition | Colletotrichum lindemuthianum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
hexa-N-acetyl-chitohexaose | pH 8.0, 50°C, recombinant enzyme | Colletotrichum lindemuthianum | |
| 5.7 | - |
penta-N-acetyl-chitopentaose | pH 8.0, 50°C, recombinant enzyme | Colletotrichum lindemuthianum | |
| 8.9 | - |
tetra-N-acetyl-chitotetraose | pH 8.0, 50°C, recombinant enzyme | Colletotrichum lindemuthianum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates by 26% | Colletotrichum lindemuthianum | |
| additional information | Li+ and Mn2+ have no effect on enzyme activity | Colletotrichum lindemuthianum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25200 | - |
x * 25200, recombinant His6-tagged enzyme, SDS-PAGE | Colletotrichum lindemuthianum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colletotrichum lindemuthianum | Q6DWK3 | - |
- |
| Colletotrichum lindemuthianum UPS9 | Q6DWK3 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | recombinant His6-tagged enzyme | Colletotrichum lindemuthianum |
| proteolytic modification | the enzyme contains a signal sequence which is cleaved at Gln28 | Colletotrichum lindemuthianum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme 364fold from Pichia pastoris strain GS115 culture supernatant by the single step process of nickel affinity chromatography | Colletotrichum lindemuthianum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity | Colletotrichum lindemuthianum |
| 0.2 | - |
recombinant His6-tagged enzyme in Pichia pastoris cell culture supernatant | Colletotrichum lindemuthianum |
| 135.6 | - |
purified recombinant His6-tagged enzyme, substrate penta-N-acetyl-chitopentaose | Colletotrichum lindemuthianum |
| 138.5 | - |
purified recombinant His6-tagged enzyme, substrate tetra-N-acetyl-chitotetraose | Colletotrichum lindemuthianum |
| 184.4 | - |
purified recombinant His6-tagged enzyme, substrate hexa-N-acetyl-chitohexaose | Colletotrichum lindemuthianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hexa-N-acetyl-chitohexaose + H2O | - |
Colletotrichum lindemuthianum | chitohexaose + acetate | - |
? | |
| hexa-N-acetyl-chitohexaose + H2O | - |
Colletotrichum lindemuthianum UPS9 | chitohexaose + acetate | - |
? | |
| additional information | substrate specificity, overview, no activity with chitotriose | Colletotrichum lindemuthianum | ? | - |
? | |
| additional information | substrate specificity, overview, no activity with chitotriose | Colletotrichum lindemuthianum UPS9 | ? | - |
? | |
| penta-N-acetyl-chitopentaose + H2O | - |
Colletotrichum lindemuthianum | chitopentaose + acetate | - |
? | |
| penta-N-acetyl-chitopentaose + H2O | - |
Colletotrichum lindemuthianum UPS9 | chitopentaose + acetate | - |
? | |
| tetra-N-acetyl-chitotetraose + H2O | - |
Colletotrichum lindemuthianum | chitotetraose + acetate | - |
? | |
| tetra-N-acetyl-chitotetraose + H2O | - |
Colletotrichum lindemuthianum UPS9 | chitotetraose + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25200, recombinant His6-tagged enzyme, SDS-PAGE | Colletotrichum lindemuthianum |
| More | comparison of enzyme amino acid sequences of strain UPS9 with those of strain ATCC 56676, overview | Colletotrichum lindemuthianum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDA | - |
Colletotrichum lindemuthianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
with substrate tetra-N-acetyl-chitotetraose | Colletotrichum lindemuthianum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 75 | half-maximal activity at 40°C and 75°C, 30% of maximal activity at 80°C | Colletotrichum lindemuthianum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life: 1 h | Colletotrichum lindemuthianum |
| 90 | - |
half-life: 30 min | Colletotrichum lindemuthianum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
with substrate tetra-N-acetyl-chitotetraose | Colletotrichum lindemuthianum |
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