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Literature summary for 3.5.1.41 extracted from
- Expression, purification, and characterization of a cobalt-activated chitin deacetylase (Cda2p) from Saccharomyces cerevisiae (2002), Protein Expr. Purif., 24, 111-116.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetate | 50 mM, 50% loss of activity | Saccharomyces cerevisiae |  |
| Ca2+ | 1-100 mM | Saccharomyces cerevisiae | |
| Cu2+ | 1-100 mM | Saccharomyces cerevisiae | |
| EDTA | - |
Saccharomyces cerevisiae | |
| Mg2+ | 1-100 mM | Saccharomyces cerevisiae | |
| additional information | N-deglycosylation of the enzyme causes total loss of activity, which can be restored by addition of 1 mM CoCl2 | Saccharomyces cerevisiae | |
| Zn2+ | 1-100 mM | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
(GlcNAc)4 | pH 8.0, 50°C | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | increases activity from 1-50 mM. 160% relative activity at 1 mM. Deglycosylation of the enzyme causes total loss of activity, which can be restored by addition of CoCl2 | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 18% carbohydrate by weight. Only N-linked glycans exist | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 771 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O | 39% deacetylation | Saccharomyces cerevisiae | ? | - |
? | |
| N,N',N'',N'''-tetraacetylchitotetraose + H2O | 22% deacetylation | Saccharomyces cerevisiae | ? | - |
? | |
| N,N',N''-triacetylchitotriose + H2O | 29% deacetylation | Saccharomyces cerevisiae | ? | - |
? | |
| N,N'-diacetylchitobiose + H2O | 17% deacetylation | Saccharomyces cerevisiae | deacetylated chitooligosaccharides + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cda2p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
24 h, 40% loss of activity | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Saccharomyces cerevisiae |
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