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Literature summary for 3.5.1.39 extracted from
- Alkylamidase of sheep liver (1971), Biochim. Biophys. Acta, 250, 216-223.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| O,O-diethyl p-nitrophenyl phosphate | inhibition at 0.001 mM | Ovis aries aries |  |
| p-chloromercuribenzoate | 20% inhibition at 1 mM | Ovis aries aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
N,N-dimethylcaproamide | inhibition by excess of substrate | Ovis aries aries | |
| 0.66 | - |
N-methylcaproamide | inhibition by excess of substrate | Ovis aries aries | |
| 0.83 | - |
N-ethylcaproamide | inhibition by excess of substrate | Ovis aries aries | |
| 1.25 | - |
N-methylheptylamide | inhibition by excess of substrate | Ovis aries aries | |
| 1.8 | - |
N-propylcaproamide | inhibition by excess of substrate | Ovis aries aries | |
| 2.2 | - |
N-Butylcaproamide | inhibition by excess of substrate | Ovis aries aries | |
| 2.5 | - |
N-phenylcaproamide | inhibition by excess of substrate | Ovis aries aries | |
| 3.7 | - |
N-methylcaprylamide | inhibition by excess of substrate | Ovis aries aries | |
| 5 | - |
N-methylvaleramide | inhibition by excess of substrate | Ovis aries aries | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Ovis aries aries | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 230000 | 250000 | gel filtration | Ovis aries aries |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 2step chromatography | Ovis aries aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Ovis aries aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.12 | - |
- |
Ovis aries aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N-dimethylcaproamide + H2O | - |
Ovis aries aries | dimethylamine + hexanoate | - |
? | |
| N-butylcaproamide + H2O | - |
Ovis aries aries | butylamine + hexanoate | - |
? | |
| N-ethylcaproamide + H2O | - |
Ovis aries aries | ethylamine + hexanoate | - |
? | |
| N-methylbutyramide + H2O | - |
Ovis aries aries | methylamine + butyric acid | - |
? | |
| N-methylcaproamide + H2O | - |
Ovis aries aries | methylamine + hexanoate | - |
? | |
| N-methylcaprylamide + H2O | low activity | Ovis aries aries | methylamine + octanoate | - |
? | |
| N-methylheptylamide + H2O | - |
Ovis aries aries | methylamine + heptanoate | - |
? | |
| N-methylvaleramide + H2O | unable to hydrolyze lower members of N-methyl substituted amides | Ovis aries aries | methylamine + pentanoate | - |
? | |
| N-phenylcaproamide + H2O | - |
Ovis aries aries | phenylamine + hexanoate | - |
? | |
| N-propylcaproamide + H2O | - |
Ovis aries aries | propylamine + hexanoate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Ovis aries aries |
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Release 2023.1 (January 2023)
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