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Literature summary for 3.5.1.30 extracted from
- delta-Aminovaleramidase of Pseudomonas putida (1970), J. Biol. Chem., 245, 3091-3096.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithioerythritol | effectively reverses heavy metal inactivation | Pseudomonas putida |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | 0.00125 mM, 100% inhibition | Pseudomonas putida | |
| arsenite | characteristic of enzymes with essential vicinal sulfhydryl groups | Pseudomonas putida | |
| Ba2+ | 5 mM, inhibition from 10-40% | Pseudomonas putida | |
| Ca2+ | 5 mM, inhibition from 10-40% | Pseudomonas putida | |
| Cd(acetate)2 | 0.00125 mM, 94% inhibition | Pseudomonas putida | |
| Cd2+ | 0.5 mM, complete inhibition , characteristic of enzymes with essential vicinal sulfhydryl groups | Pseudomonas putida | |
| Co2+ | 5.0 mM,75% inhibition | Pseudomonas putida | |
| Cu2+ | 5.0 mM, complete inhibition | Pseudomonas putida | |
| Fe2+ | 5 mM, inhibition from 10-40% | Pseudomonas putida | |
| Hg(NO3)2 | 0.187 mM, 100% inhibition | Pseudomonas putida | |
| Hg2+ | 0.5 mM, complete inhibition | Pseudomonas putida | |
| HgCl2 | 0.00125 mM, 100% inhibition | Pseudomonas putida | |
| Mg2+ | 5 mM, inhibition from 10-40% | Pseudomonas putida | |
| NaAsO2 | 0.0125 mM, 47% inhibition | Pseudomonas putida | |
| p-hydroxymercuribenzoate | 0.00025 mM, 44% inhibition, inhibition partially reversed by dithioerythritol | Pseudomonas putida | |
| Pb2+ | 5.0 mM, complete inhibition | Pseudomonas putida | |
| Sn2+ | 5.0 mM, complete inhibition | Pseudomonas putida | |
| Zn2+ | 0.5 mM, complete inhibition | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
6-Aminohexanamide | - |
Pseudomonas putida | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 0.125 mM stimulates activity 37% | Pseudomonas putida | |
| Fe3+ | 0.125 mM stimulates activity 25% | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-aminopentanamide + H2O | Pseudomonas putida | known intermediate in lysine catabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified 400fold from the soluble fraction of cell-free extract | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 130 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-aminopentanamide + H2O | - |
Pseudomonas putida | 5-aminopentanoate + NH3 | - |
? | |
| 5-aminopentanamide + H2O | known intermediate in lysine catabolism | Pseudomonas putida | ? | - |
? | |
| 6-aminohexanamide + H2O | - |
Pseudomonas putida | 6-aminohexanoate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| delta-aminovaleramidase | - |
Pseudomonas putida |
| delta-aminovaleramide amidohydrolase | - |
Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | stable below pH 6.5, irreversibly inactivated above pH 9 | Pseudomonas putida |
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