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Literature summary for 3.5.1.26 extracted from

  • Wang, Y.; Guo, H.C.
    Crystallographic snapshot of a productive glycosylasparaginase-substrate complex (2007), J. Mol. Biol., 366, 82-92.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.26

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant with lower turnover number to be able to get a crystal with natural substrate in complex Elizabethkingia meningoseptica

Protein Variants

Protein Variants Comment Organism
T152C mutant with lower turnover number to get a crystall in complex with natural substrate Elizabethkingia meningoseptica

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Elizabethkingia meningoseptica 5764
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N4-(beta-N-acetylglucosaminyl)-L-asparagine + H2O Elizabethkingia meningoseptica deficiency in the activity of human GA leads to a lysosomal storage disease aspartylglycosaminuria 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Elizabethkingia meningoseptica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O
-
 Elizabethkingia meningoseptica N-acetyl-beta-D-glucosaminylamine + L-aspartate
-
 ?
N4-(beta-N-acetylglucosaminyl)-L-asparagine + H2O deficiency in the activity of human GA leads to a lysosomal storage disease aspartylglycosaminuria Elizabethkingia meningoseptica 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 ?

Subunits

Subunits Comment Organism
homodimer autoproteolysis results in a 17000 Da alpha and a 15000 beta subunit, together both dimers form an alpha-beta-beta-alpha sandwich Elizabethkingia meningoseptica

Synonyms

Synonyms Comment Organism
glycosylasparaginase
-
 Elizabethkingia meningoseptica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00015
-
 N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine mutant with T152C Elizabethkingia meningoseptica
0.00015
-
 N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine mutant enzyme T152C Elizabethkingia meningoseptica
16
-
 N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine wild type enzyme Elizabethkingia meningoseptica
16
-
 N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine native enzyme Elizabethkingia meningoseptica