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Literature summary for 3.5.1.26 extracted from

  • Liu, Y.; Dunn, G.S.; Aronson, N.N.
    Purification, biochemistry and molecular cloning of an insect glycosylasparaginase from Spodoptera frugiperda (1996), Glycobiology, 6, 527-536.
    View publication on PubMed
Search for more literature for 3.5.1.26

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.88
-
 N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
-
 Spodoptera frugiperda

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 alpha 1, 1 * 28000 + beta 1, 1 * 17000, SDS-PAGE Spodoptera frugiperda
17000
-
 alpha2,beta2, 2 * 28000 + 2 * 17000, SDS-PAGE Spodoptera frugiperda
28000
-
 alpha 1, 1 * 28000 + beta 1, 1 * 17000, SDS-PAGE Spodoptera frugiperda
28000
-
 alpha2,beta2, 2 * 28000 + 2 * 17000, SDS-PAGE Spodoptera frugiperda
47000
-
 gel filtration Spodoptera frugiperda
101000
-
 gel filtration Spodoptera frugiperda

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Spodoptera frugiperda
-
 L-aspartate + NH3
-
 ?
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O Bos taurus
-
 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 ?
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O Spodoptera frugiperda
-
 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 ?
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O Rattus sp.
-
 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus sp.
-
-
-
Spodoptera frugiperda
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Spodoptera frugiperda

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Bos taurus
-
placenta
-
 Rattus sp.
-
SF-9 cell
-
 Spodoptera frugiperda
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
-
 Spodoptera frugiperda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Spodoptera frugiperda L-aspartate + NH3
-
 ?
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O
-
 Bos taurus 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 ?
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O
-
 Spodoptera frugiperda 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 ?
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O
-
 Rattus sp. 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Bos taurus
dimer
-
 Rattus sp.
dimer alpha 1, 1 * 28000 + beta 1, 1 * 17000, SDS-PAGE Spodoptera frugiperda
tetramer alpha2,beta2, 2 * 28000 + 2 * 17000, SDS-PAGE Spodoptera frugiperda

Synonyms

Synonyms Comment Organism
glycosylasparaginase
-
 Spodoptera frugiperda

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65 70 denaturation Spodoptera frugiperda

pH Range

pH Minimum pH Maximum Comment Organism
4 10.5
-
 Spodoptera frugiperda