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Literature summary for 3.5.1.25 extracted from

  • Hall, R.S.; Xiang, D.F.; Xu, C.; Raushel, F.M.
    N-Acetyl-D-glucosamine-6-phosphate deacetylase: substrate activation via a single divalent metal ion (2007), Biochemistry, 46, 7942-7952.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.25

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 N-acetyl-D-glucosamine 6-phosphate
-
 Escherichia coli
0.08
-
 N-acetyl-D-glucosamine 6-phosphate mutant Q59A Escherichia coli
0.1
-
 N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Mn Escherichia coli
0.15
-
 N-acetyl-D-glucosamine 6-phosphate mutant E131A Escherichia coli
0.15
-
 N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Co Escherichia coli
0.2
-
 N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Cd Escherichia coli
0.2
-
 N-thioacetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Cd Escherichia coli
0.23
-
 N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Fe Escherichia coli
0.23
-
 N-thioacetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Mn Escherichia coli
0.24
-
 N-thioacetyl-D-glucosamine 6-phosphate
-
 Escherichia coli
0.29
-
 N-formyl-D-glucosamine 6-phosphate
-
 Escherichia coli
0.31
-
 N-acetyl-D-glucosamine 6-phosphate mutant Q59H Escherichia coli
0.33
-
 N-acetyl-D-glucosamine 6-phosphate mutant H143Q Escherichia coli
0.4
-
 N-trifluoroacetyl-D-glucosamine 6-phosphate
-
 Escherichia coli
0.64
-
 N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Ni Escherichia coli
0.76
-
 N-acetyl-D-glucosamine 6-phosphate mutant Y223F Escherichia coli
0.8
-
 N-acetyl-D-glucosamine 6-phosphate mutant N61A Escherichia coli
1.24
-
 N-Acetyl-D-galactosamine 6-phosphate
-
 Escherichia coli
1.7
-
 N-acetyl-D-glucosamine 6-phosphate mutant N61H Escherichia coli
2
-
 N-acetyl-D-glucosamine 6-phosphate mutant K139M Escherichia coli
2.1
-
 N-acetyl-D-glucosamine 6-phosphate mutant H143N Escherichia coli
2.7
-
 N-acetyl-D-glucosamine 6-phosphate mutant H251N Escherichia coli
3.6
-
 N-acetyl-D-glucosamine 6-phosphate mutant E131Q Escherichia coli
4.9
-
 N-acetyl-D-glucosamine 6-sulfate
-
 Escherichia coli
11
-
 N-acetyl-D-glucosamine 6-sulfate Zn in acitve site changed to Cd Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn one cation per subunit Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AF18
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange column Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-galactosamine 6-phosphate + H2O
-
 Escherichia coli D-galactosamine 6-phosphate + acetate
-
 ?
N-acetyl-D-glucosamine 6-phosphate + H2O
-
 Escherichia coli D-glucosamine 6-phosphate + acetate
-
 ?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
 Escherichia coli D-glucosamine 6-sulfate + acetate
-
 ?
N-formyl-D-glucosamine 6-phosphate + H2O
-
 Escherichia coli D-glucosamine 6-phosphate + formate
-
 ?
N-thioacetyl-D-glucosamine 6-phosphate + H2O
-
 Escherichia coli D-glucosamine 6-phosphate + thioacetate
-
 ?
N-trifluoroacetyl-D-glucosamine 6-phosphate + H2O
-
 Escherichia coli D-glucosamine 6-phosphate + trifluoroacetate
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
N-acetyl-D-glucosamine-6-phosphate deacetylase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 N-acetyl-D-glucosamine 6-phosphate mutant D273A Escherichia coli
0.02
-
 N-acetyl-D-glucosamine 6-phosphate mutant D273N Escherichia coli
0.43
-
 N-acetyl-D-glucosamine 6-phosphate mutant H143N Escherichia coli
0.7
-
 N-acetyl-D-glucosamine 6-phosphate mutant E131Q Escherichia coli
1.9
-
 N-acetyl-D-glucosamine 6-phosphate mutant E131A Escherichia coli
2.4
-
 N-acetyl-D-glucosamine 6-phosphate mutant H143Q Escherichia coli
2.6
-
 N-acetyl-D-glucosamine 6-phosphate mutant N61H Escherichia coli
6.8
-
 N-acetyl-D-glucosamine 6-phosphate mutant H251N Escherichia coli
10
-
 N-thioacetyl-D-glucosamine 6-phosphate
-
 Escherichia coli
10
-
 N-acetyl-D-glucosamine 6-phosphate mutant Q59A Escherichia coli
11
-
 N-thioacetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Mn Escherichia coli
22
-
 N-formyl-D-glucosamine 6-phosphate
-
 Escherichia coli
23
-
 N-acetyl-D-glucosamine 6-sulfate Zn in acitve site changed to Cd Escherichia coli
24
-
 N-acetyl-D-glucosamine 6-phosphate mutant N61A Escherichia coli
32
-
 N-acetyl-D-glucosamine 6-phosphate mutant Q59H Escherichia coli
35
-
 N-acetyl-D-glucosamine 6-phosphate enzyme with 0.4 eqivalents of Zn per subunit bound Escherichia coli
41
-
 N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Ni Escherichia coli
49
-
 N-acetyl-D-glucosamine 6-phosphate mutant K139M Escherichia coli
58
-
 N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Fe Escherichia coli
64
-
 N-acetyl-D-glucosamine 6-sulfate
-
 Escherichia coli
92
-
 N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Mn Escherichia coli
96
-
 N-acetyl-D-glucosamine 6-phosphate enzyme with 1 equivalent of Zn per subunit bound Escherichia coli
128
-
 N-thioacetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Cd Escherichia coli
154
-
 N-Acetyl-D-galactosamine 6-phosphate
-
 Escherichia coli
163
-
 N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Cd Escherichia coli
167
-
 N-acetyl-D-glucosamine 6-phosphate mutant Y223F Escherichia coli
177
-
 N-acetyl-D-glucosamine 6-phosphate Zn in acitve site changed to Co Escherichia coli
2610
-
 N-trifluoroacetyl-D-glucosamine 6-phosphate
-
 Escherichia coli