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Literature summary for 3.5.1.25 extracted from

  • Yamano, N.; Matsushita, Y.; Kamada, K.; Fujishima, S.; Arita, M.
    Purification and characterization of N-acetylglucosamine 6-phosphate deacetylase with activity against N-acetylglucosamine from Vibrio cholera Non-O1 (1996), Biosci. Biotechnol. Biochem., 60, 1320-1323.
    View publication on PubMed
Search for more literature for 3.5.1.25

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, complete inhibition Vibrio cholerae serotype O1
EDTA 1 mM, 25% inhibition Vibrio cholerae serotype O1
NEM 1 mM, complete inhibition Vibrio cholerae serotype O1
PCMB 0.1 mM, complete inhibition Vibrio cholerae serotype O1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 N-acetylglucosamine 6-phosphate
-
 Vibrio cholerae serotype O1
4.6
-
 N-acetylglucosamine 6-sulfate
-
 Vibrio cholerae serotype O1
73
-
 N-acetylglucosamine
-
 Vibrio cholerae serotype O1

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 200% activation at 4 mM Vibrio cholerae serotype O1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 4 * 45000, SDS-PAGE Vibrio cholerae serotype O1
190000
-
 gel filtration Vibrio cholerae serotype O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
 Non-O1
-
Vibrio cholerae serotype O1 Non-O1
-
 Non-O1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio cholerae serotype O1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.8
-
-
 Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-glucosamine 6-phosphate + H2O
-
 Vibrio cholerae serotype O1 D-glucosamine 6-phosphate + acetate
-
 ?
N-acetyl-D-glucosamine 6-phosphate + H2O
-
 Vibrio cholerae serotype O1 Non-O1 D-glucosamine 6-phosphate + acetate
-
 ?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
 Vibrio cholerae serotype O1 D-glucosamine 6-sulfate + acetate
-
 ?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
 Vibrio cholerae serotype O1 Non-O1 D-glucosamine 6-sulfate + acetate
-
 ?
N-acetylglucosamine + H2O
-
 Vibrio cholerae serotype O1 D-glucosamine + acetate
-
 ?
N-acetylglucosamine + H2O
-
 Vibrio cholerae serotype O1 Non-O1 D-glucosamine + acetate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 45000, SDS-PAGE Vibrio cholerae serotype O1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Vibrio cholerae serotype O1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
29
-
 pH 6.0-8.0, 30 min, stable up to Vibrio cholerae serotype O1
30
-
 pH 6.0-8.0, 30 min, enzyme retains more than 70% of its activity Vibrio cholerae serotype O1
45
-
 pH 6.0-8.0, 30 min, complete loss of activity Vibrio cholerae serotype O1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.97
-
 N-acetylglucosamine 6-phosphate
-
 Vibrio cholerae serotype O1
25
-
 N-acetylglucosamine
-
 Vibrio cholerae serotype O1
50
-
 N-acetylglucosamine 6-sulfate
-
 Vibrio cholerae serotype O1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.5
-
 Vibrio cholerae serotype O1

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 30 min, stable up to 29°C, enzyme retains more than 70% of its activity at 30°C, complete loss of activity at 45°C Vibrio cholerae serotype O1