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Literature summary for 3.5.1.24 extracted from

  • Chae, J.P.; Valeriano, V.D.; Kim, G.B.; Kang, D.K.
    Molecular cloning, characterization and comparison of bile salt hydrolases from Lactobacillus johnsonii PF01 (2013), J. Appl. Microbiol., 114, 121-133.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene PF01_00515, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged isozyme in Escherichia coli strain BLR(DE3) Lactobacillus johnsonii
gene PF01_00682, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged isozyme in Escherichia coli strain BLR(DE3) Lactobacillus johnsonii
gene PF01_01578, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged isozyme in Escherichia coli strain BLR(DE3) Lactobacillus johnsonii

Organism

Organism UniProt Comment Textmining
Lactobacillus johnsonii A0A1B3PS02 isozyme BSH A; formerly Lactobacillus acidophilus strain PF01, gene PF01_00515, isozyme BSH A
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Lactobacillus johnsonii A0A4S2CHH9 isozyme BSH B; formerly Lactobacillus acidophilus strain PF01, gene PF01_01578, isozyme BSH B
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Lactobacillus johnsonii A0A4Z0GFP0 BSH C; formerly Lactobacillus acidophilus strain PF01, gene PF01_00682, isozyme BSH C
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Lactobacillus johnsonii PF01 A0A1B3PS02 isozyme BSH A; formerly Lactobacillus acidophilus strain PF01, gene PF01_00515, isozyme BSH A
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Lactobacillus johnsonii PF01 A0A4S2CHH9 isozyme BSH B; formerly Lactobacillus acidophilus strain PF01, gene PF01_01578, isozyme BSH B
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Lactobacillus johnsonii PF01 A0A4Z0GFP0 BSH C; formerly Lactobacillus acidophilus strain PF01, gene PF01_00682, isozyme BSH C
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography Lactobacillus johnsonii

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isoyzme BSH A hydrolyses tauro-conjugated bile salts, whereas isozyme BSH C hydrolyses glycoconjugated bile salts. The enzymes have no preferential activities towards a specific cholyl moiety, the isozymes differ in their substrate specificities, overview Lactobacillus johnsonii ?
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?
additional information isoyzme BSH A hydrolyses tauro-conjugated bile salts, whereas isozyme BSH C hydrolyses glycoconjugated bile salts. The enzymes have no preferential activities towards a specific cholyl moiety, the isozymes differ in their substrate specificities, overview Lactobacillus johnsonii PF01 ?
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?

Synonyms

Synonyms Comment Organism
bile salt hydrolase
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Lactobacillus johnsonii
BSH
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Lactobacillus johnsonii
BSH A
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Lactobacillus johnsonii
BSH B
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Lactobacillus johnsonii
BSH C
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Lactobacillus johnsonii
conjugated bile salt hydrolase UniProt Lactobacillus johnsonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
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isozyme BSH A Lactobacillus johnsonii
70
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isozyme BSH C Lactobacillus johnsonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
isozyme BSH A Lactobacillus johnsonii
5
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isozyme BSH C Lactobacillus johnsonii