Literature summary for 3.5.1.24 extracted from

Rossocha, M.; Schultz-Heienbrok, R.; von Moeller, H.; Coleman, J.P.; Saenger, W.
Conjugated bile acid hydrolase is a tetrameric N-terminal thiol hydrolase with specific recognition of its cholyl but not of its tauryl product (2005), Biochemistry, 44, 5739-5748.

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with substrate taurodeoxycholate and as apoenzyme, complexed enzyme by hanging drop vapour diffusion method, 18°C, mixing of 0.003 ml protein solution and 0.002 ml reservoir solution, the latter containing 2.6 M ammonium sulfate, 0.1 M sodium citrate, pH 6.0, and 1 mM taurodeoxycholate, 3 days, apoenzyme crystals by sitting drop vapour diffusion method, 18°C, mixing of 0.0015 ml protein solution with 0.001 ml reservoir solution containing 25% PEG 4000, 0.2 M ammonium sulfate, and 0.1 M Bis-Tris, pH 5.5, 3 days, orthorhombic crystals in both cases, X-ray diffraction structure determination and analysis at 1.7 and 2.1 A resolution, respectively	Clostridium perfringens

Crystallization (Comment)

Organism

enzyme in complex with substrate taurodeoxycholate and as apoenzyme, complexed enzyme by hanging drop vapour diffusion method, 18°C, mixing of 0.003 ml protein solution and 0.002 ml reservoir solution, the latter containing 2.6 M ammonium sulfate, 0.1 M sodium citrate, pH 6.0, and 1 mM taurodeoxycholate, 3 days, apoenzyme crystals by sitting drop vapour diffusion method, 18°C, mixing of 0.0015 ml protein solution with 0.001 ml reservoir solution containing 25% PEG 4000, 0.2 M ammonium sulfate, and 0.1 M Bis-Tris, pH 5.5, 3 days, orthorhombic crystals in both cases, X-ray diffraction structure determination and analysis at 1.7 and 2.1 A resolution, respectively

Clostridium perfringens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
taurodeoxycholate + H2O	Clostridium perfringens	-	taurine + deoxycholate	-	?

Organism

Organism	UniProt	Comment	Textmining
Clostridium perfringens	P54965	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
glycocholate + H2O = cholate + glycine	substrate binding pocket and active site structure, taurine has a reversed orientation pointing with its sulfo group towards Cys2 and leaving the active site with its amino group ahead, overview	Clostridium perfringens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
taurodeoxycholate + H2O	-	Clostridium perfringens	taurine + deoxycholate	-	?

Subunits

Subunits	Comment	Organism
More	computational structure comparisons	Clostridium perfringens
tetramer	the enzyme contains an N-terminal thiol hydrolase activity site, quarternary structure, crystal structure analysis, overview	Clostridium perfringens

Synonyms

Synonyms	Comment	Organism
bile salt hydrolase	-	Clostridium perfringens
CBAH	-	Clostridium perfringens
Conjugated bile acid hydrolase	-	Clostridium perfringens