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Literature summary for 3.5.1.22 extracted from
- Pantothenase from Pseudomonas fluorescens (1979), Methods Enzymol., 62, 267-275.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oxalate | - |
Pseudomonas fluorescens |  |
| oxaloacetate | noncompetitive inhibition, dependent on pH | Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.6 | - |
pantothenic acid | pH-independent theoretical Km | Pseudomonas fluorescens | |
| 15 | - |
pantothenic acid | dependent on pH, phosphate buffer, pH 7.0, 20°C | Pseudomonas fluorescens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Pseudomonas fluorescens | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Pseudomonas fluorescens |
| 97400 | - |
gel filtration | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pantothenic acid + H2O | Pseudomonas fluorescens | sole source of carbon and nitrogen | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.8 | - |
- |
Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pantothenate + H2O | - |
Pseudomonas fluorescens | 2,4-dihydroxy-3,3-dimethylbutyric acid + beta-alanine | - |
? | |
| pantothenic acid + H2O | - |
Pseudomonas fluorescens | beta-alanine + D-pantoyl lactone + H2O | - |
r | |
| pantothenic acid + H2O | - |
Pseudomonas fluorescens | beta-alanine + D-pantoic acid | - |
ir | |
| pantothenic acid + H2O | sole source of carbon and nitrogen | Pseudomonas fluorescens | ? | - |
? | |
| pantoyl-gamma-aminobutyrate + H2O | - |
Pseudomonas fluorescens | 4-aminobutyrate + D-pantoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | above 30°C rapidly inactivated, at 35°C inactivation rate 7.2%/min | Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | - |
Pseudomonas fluorescens |
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