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Literature summary for 3.5.1.20 extracted from

  • Park, B.S.; Hirotani, A.; Nakano, Y.; Kitaoka, S.
    Subcellular distribution and some properties of citrullinase in Euglena gracilis Z (1985), Agric. Biol. Chem., 49, 2205-2206.
No PubMed abstract available
Search for more literature for 3.5.1.20

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 1 mM, inhibition reversed by 2-mercaptoethanol Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.06
-
 citrulline
-
 Tetrahymena sp.
4.55
-
 citrulline
-
 Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Euglena gracilis 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 3 * 31000, 3 identical subunits, SDS-PAGE Euglena gracilis
94000
-
 gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-citrulline + H2O Tetrahymena sp. first intermediate in urea cycle, involved in arginine biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
 protozoan, strain Z
-
Tetrahymena sp.
-
 protozoan
-
Tetrahymena sp.
-
 thermophila, pyryformis
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tetrahymena sp.
64fold Euglena gracilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0334
-
-
 Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrulline + H2O
-
 Euglena gracilis ornithine + CO2 + NH3
-
 ?
citrulline + H2O
-
 Tetrahymena sp. ornithine + CO2 + NH3
-
 ?
L-citrulline + H2O first intermediate in urea cycle, involved in arginine biosynthesis Tetrahymena sp. ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 31000, 3 identical subunits, SDS-PAGE Euglena gracilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 stable up to 40°C for 10 min, retains 87% activity after treatment at 50°C, activity completely lost at 60°C Euglena gracilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 stable up to 40°C for 10 min, retains 87% activity after treatment at 50°C, activity completely lost at 60°C Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Tetrahymena sp.
7 7.2
-
 Euglena gracilis