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Literature summary for 3.5.1.2 extracted from

  • Ito, K.; Umitsuki, G.; Oguma, T.; Koyama, Y.
    Salt-tolerant and thermostable glutaminases of Cryptococcus species form a new glutaminase family (2011), Biosci. Biotechnol. Biochem., 75, 1317-1324.
    View publication on PubMed
Search for more literature for 3.5.1.2

Cloned(Commentary)

Cloned (Comment) Organism
gene CagahA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Saccharomyces cerevisiae strain INVSc Naganishia albida
gene CngahA, a single copy gene in the chromosomal DNA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Saccharomyces cerevisiae strain INVSc Papiliotrema flavescens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Naganishia albida 9986
-
cell surface
-
 Papiliotrema flavescens 9986
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73801
-
 x * 78000 recombinant enzyme, SDS-PAGEm x * 73801, sequence calculation Papiliotrema flavescens
78000
-
 x * 78000 recombinant enzyme, SDS-PAGEm x * 73801, sequence calculation Papiliotrema flavescens
270000
-
 recombinant enzyme, gel filtration Papiliotrema flavescens

Organism

Organism UniProt Comment Textmining
Naganishia albida
-
 gene CagahA
-
Naganishia albida ATCC 20293
-
 gene CagahA
-
Papiliotrema flavescens
-
 gene CngahA
-
Papiliotrema flavescens NISL 3771
-
 gene CngahA
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant enzyme Papiliotrema flavescens

Subunits

Subunits Comment Organism
homotrimer or homotetramer x * 78000 recombinant enzyme, SDS-PAGEm x * 73801, sequence calculation Papiliotrema flavescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Naganishia albida
37
-
 assay at Papiliotrema flavescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Naganishia albida
7
-
 assay at Papiliotrema flavescens

General Information

General Information Comment Organism
evolution the enzyme belongs to a distinct type of glutaminase with an amidase signature sequence forming a distinct family Naganishia albida
evolution the enzyme belongs to a distinct type of glutaminase with an amidase signature sequence forming a distinct family Papiliotrema flavescens