Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2 mM, 2fold activation | Acidilobus saccharovorans | |
dithiothreitol | 2 mM, 2.1fold activation | Acidilobus saccharovorans | |
EDTA | 57% inhibitioin at 10 mM. 10% inhibition at 1 mM | Acidilobus saccharovorans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acidilobus saccharovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 1 mM, 94% inhibition | Acidilobus saccharovorans | |
NaCl | 50 mM, 12% inhibition | Acidilobus saccharovorans | |
Zn2+ | 5 mM, complete inhibition | Acidilobus saccharovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
nicotinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
0.41 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains Fe2+ (0.77 mol per mol of protein). 1.6fold activation by 5 mM Fe2+ | Acidilobus saccharovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46400 | - |
- |
Acidilobus saccharovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidilobus saccharovorans | D9Q1R5 | - |
- |
Acidilobus saccharovorans DSM 16705 | D9Q1R5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with N-carbamoylsarcosine, hydantoic acid, acrylamide, D- and L-asparagine, D- and L-glutamine | Acidilobus saccharovorans | ? | - |
? | |
additional information | no activity with N-carbamoylsarcosine, hydantoic acid, acrylamide, D- and L-asparagine, D- and L-glutamine | Acidilobus saccharovorans DSM 16705 | ? | - |
? | |
nicotinamide + H2O | - |
Acidilobus saccharovorans | nicotinate + NH3 | - |
? | |
nicotinamide + H2O | - |
Acidilobus saccharovorans DSM 16705 | nicotinate + NH3 | - |
? | |
pyrazinamide + H2O | - |
Acidilobus saccharovorans | pyrazine-2-carboxylic acid + NH3 | - |
? | |
pyrazinamide + H2O | - |
Acidilobus saccharovorans DSM 16705 | pyrazine-2-carboxylic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | in solution the protein exists as a dimer with a small admixture of tetrameric form | Acidilobus saccharovorans |
Synonyms | Comment | Organism |
---|---|---|
ASAC_0847 | - |
Acidilobus saccharovorans |
NA_As0847 | - |
Acidilobus saccharovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Acidilobus saccharovorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 100 | 60°C: about 45% of maximal activity, 100°C: 85% of maximal activity | Acidilobus saccharovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
t1/2: 180 min | Acidilobus saccharovorans |
80 | - |
t1/2: 35 min | Acidilobus saccharovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
76.9 | - |
nicotinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
135.7 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Acidilobus saccharovorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | hydrolysis of nicotinamide of a wide range of pH values | Acidilobus saccharovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
331 | - |
Pyrazinamide | pH 7.5, 60°C | Acidilobus saccharovorans | |
427 | - |
nicotinamide | pH 7.5, 60°C | Acidilobus saccharovorans |