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Literature summary for 3.5.1.19 extracted from

  • French, J.B.; Cen, Y.; Sauve, A.A.; Ealick, S.E.
    High-resolution crystal structures of Streptococcus pneumoniae nicotinamidase with trapped intermediates provide insights into the catalytic mechanism and inhibition by aldehydes (2010), Biochemistry, 49, 8803-8812.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) pLysS cells Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
a trapped nicotinoyl-thioester complexed with wild type enzyme and C136S mutant in complex with nicotinamide, hanging drop vapor diffusion method, using 18-22% (w/v) polyethylene glycol 3350, 0.2-0.3 M NaCl and 0.2 M sodium malonate, pH 6.3, at 18°C Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
C136S inactive Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
5-methoxynicotinaldehyde competitive inhibitor Streptococcus pneumoniae
nicotinaldehyde competitive inhibitor Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ addition of Co2+ after metal removement results in 13% activity Streptococcus pneumoniae
Fe2+ addition of Fe2+ after metal removement results in 13% activity Streptococcus pneumoniae
Mn2+ addition of Mn2+ after metal removement results in 27% activity Streptococcus pneumoniae
additional information addition of Fe3+ or Ni2+ after metal removement results in no detectable activity Streptococcus pneumoniae
Zn2+ contains zinc, highest level of activity in the presence of Zn2+ Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21400
-
4 * 21400, calculated from amino acid sequence Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinamide + H2O Streptococcus pneumoniae
-
nicotinate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae A0A0H2UR34
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinamide + H2O
-
Streptococcus pneumoniae nicotinate + NH3
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 21400, calculated from amino acid sequence Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
NIC
-
Streptococcus pneumoniae