Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2(DE3) pLysS cells | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
a trapped nicotinoyl-thioester complexed with wild type enzyme and C136S mutant in complex with nicotinamide, hanging drop vapor diffusion method, using 18-22% (w/v) polyethylene glycol 3350, 0.2-0.3 M NaCl and 0.2 M sodium malonate, pH 6.3, at 18°C | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
C136S | inactive | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-methoxynicotinaldehyde | competitive inhibitor | Streptococcus pneumoniae | |
nicotinaldehyde | competitive inhibitor | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | addition of Co2+ after metal removement results in 13% activity | Streptococcus pneumoniae | |
Fe2+ | addition of Fe2+ after metal removement results in 13% activity | Streptococcus pneumoniae | |
Mn2+ | addition of Mn2+ after metal removement results in 27% activity | Streptococcus pneumoniae | |
additional information | addition of Fe3+ or Ni2+ after metal removement results in no detectable activity | Streptococcus pneumoniae | |
Zn2+ | contains zinc, highest level of activity in the presence of Zn2+ | Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21400 | - |
4 * 21400, calculated from amino acid sequence | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | Streptococcus pneumoniae | - |
nicotinate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | A0A0H2UR34 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | - |
Streptococcus pneumoniae | nicotinate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 21400, calculated from amino acid sequence | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
NIC | - |
Streptococcus pneumoniae |