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Literature summary for 3.5.1.18 extracted from
- Kinetic and spectroscopic characterization of the E134A- and E134D-altered dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae (2006), J. Biol. Inorg. Chem., 11, 206-216.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Haemophilus influenzae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E134A | Glu134 replaced by alanine | Haemophilus influenzae |
| E134D | Glu134 replaced by aspartate | Haemophilus influenzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
N-succinyl-LL-diaminopimelate | mutant enzyme E134D at pH 7.5 | Haemophilus influenzae |  |
| 0.73 | - |
N-succinyl-LL-diaminopimelate | wild type enzyme at pH 7.5 | Haemophilus influenzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41500 | - |
SDS-PAGE | Haemophilus influenzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus influenzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| fast-flow Q-sepharose anionexchange column chromatography | Haemophilus influenzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.17 | - |
mutant enzyme E134D at pH 7.5 | Haemophilus influenzae |
| 180 | - |
wild type enzyme at pH 7.5 | Haemophilus influenzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-succinyl-LL-diaminopimelate + H2O | - |
Haemophilus influenzae | ? | - |
? | |
| N-succinyl-LL-diaminopimelic acid + H2O | - |
Haemophilus influenzae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DapE | - |
Haemophilus influenzae |
| dapE-encoded N-succinyl-LL-diaminopimelic acid desuccinylase | - |
Haemophilus influenzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
N-succinyl-LL-diaminopimelate | mutant enzyme E134D at pH 7.5 | Haemophilus influenzae | |
| 140 | - |
N-succinyl-LL-diaminopimelate | wild type enzyme at pH 7.5 | Haemophilus influenzae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Haemophilus influenzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | activity below pH 6.0 was not observed | Haemophilus influenzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | activity below pH 6.0 was not observed | Haemophilus influenzae |
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