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Literature summary for 3.5.1.16 extracted from

  • McGregor, W.C.; Swierczek, S.I.; Bennett, B.; Holz, R.C.
    Characterization of the catalytically active Mn(II)-loaded argE-encoded N-acetyl-L-ornithine deacetylase from Escherichia coli (2007), J. Biol. Inorg. Chem., 12, 603-613.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
[(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl]leucine bestatin, competitive inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
N-alpha-acetyl-L-ornithine in 50 mM Chelex-100 treated sodium phosphate buffer, pH 7.5, at 25°C, in the presence of Mn2+ or Zn2+ Escherichia coli
1.2
-
N-alpha-acetyl-L-ornithine in 50 mM Chelex-100 treated sodium phosphate buffer, pH 7.5, at 25°C, in the presence of Co2+ Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42350
-
calculated from amino acid sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-acetyl-L-ornithine + H2O
-
Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
ArgE
-
Escherichia coli
argE-encoded N-acetyl-L-ornithine deacetylase
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 55
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
550
-
N-alpha-acetyl-L-ornithine in 50 mM Chelex-100 treated sodium phosphate buffer, pH 7.5, at 25°C, in the presence of Mn2+ Escherichia coli
1600
-
N-alpha-acetyl-L-ornithine in 50 mM Chelex-100 treated sodium phosphate buffer, pH 7.5, at 25°C, in the presence of Zn2+ Escherichia coli
3800
-
N-alpha-acetyl-L-ornithine in 50 mM Chelex-100 treated sodium phosphate buffer, pH 7.5, at 25°C, in the presence of Co2+ Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.067
-
[(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl]leucine
-
Escherichia coli