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Literature summary for 3.5.1.16 extracted from

  • Meinnel, T.; Schmitt, E.; Mechulam, Y.; Blanquet, S.
    Structural and biochemical characterization of the Escherichia coli arg gene product (1992), J. Bacteriol., 174, 2323-2331.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
plasmid pMC7 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.56
-
acetylornithine
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
pure NAO, SDS-PAGE Escherichia coli
42350
-
calculated polypeptide, corresponding to AO nucleotide sequence Escherichia coli
52000
-
SDS-PAGE Escherichia coli
62000
-
gel filtration Escherichia coli
77000
-
nondenaturing PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N2-acety-L-ornithine + H2O Escherichia coli step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Saccharomyces sp. step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Escherichia coli 160-37 step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Escherichia coli ATCC 9637 step in arginine biosynthesis L-ornithine + acetate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12
-
Escherichia coli
-
K-37, UF121, UF121R, PAL123
-
Escherichia coli
-
160-37
-
Escherichia coli
-
ATCC9637
-
Escherichia coli 160-37
-
160-37
-
Escherichia coli ATCC 9637
-
ATCC9637
-
Saccharomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
87.1
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-DL-methionine + H2O
-
Escherichia coli methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Saccharomyces sp. methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Escherichia coli 160-37 methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Escherichia coli ATCC 9637 methionine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Saccharomyces sp. L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli 160-37 L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli ATCC 9637 L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Escherichia coli L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Saccharomyces sp. L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Escherichia coli 160-37 L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Escherichia coli ATCC 9637 L-ornithine + acetate
-
?
Nalpha-benzoyl-L-ornithine + H2O
-
Escherichia coli benzoate + ornithine
-
?
Nalpha-benzoyl-L-ornithine + H2O
-
Escherichia coli 160-37 benzoate + ornithine
-
?
Nalpha-benzoyl-L-ornithine + H2O
-
Escherichia coli ATCC 9637 benzoate + ornithine
-
?

Subunits

Subunits Comment Organism
dimer
-
Escherichia coli

Synonyms

Synonyms Comment Organism
NAO
-
Escherichia coli