Literature summary for 3.5.1.15 extracted from

Le Coq, J.; Pavlovsky, A.; Malik, R.; Sanishvili, R.; Xu, C.; Viola, R.E.
Examination of the mechanism of human brain aspartoacylase through the binding of an intermediate analogue (2008), Biochemistry, 47, 3484-3492.

Search for more literature for 3.5.1.15

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization in complex with inhibitor N-phosphonomethyl-L-aspartate, crystallization conditions: 50 mM sodium citrate (pH 6.0), 300 mM K2HPO4, and 15-19% polyethylene glycol 3350	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E178D	10% activity of native enzyme	Homo sapiens
E24A	no detectable activity	Homo sapiens
E24D	no detectable activity	Homo sapiens
E24G	no protein expression	Homo sapiens
H116A	no detectable activity	Homo sapiens
H21A	no detectable activity	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
N-phosphonomethyl-L-aspartate	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	one zinc ion per monomer	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-acetyl-L-aspartate + H2O	Homo sapiens	malfunction of the enzyme causes Canavan disease	L-aspartate + acetate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P45381	-	-

Purification (Commentary)

Purification (Comment)	Organism
anion exchange chromatography followed by size exclusion chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-acetyl-L-aspartate + H2O	malfunction of the enzyme causes Canavan disease	Homo sapiens	L-aspartate + acetate	-	?

Subunits

Subunits	Comment	Organism
homodimer	extensive contact surface area between the two subunits	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Aspartoacylase	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.3	-	N-phosphonomethyl-L-aspartate	-	Homo sapiens

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Release 2023.1 (January 2023)