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Literature summary for 3.5.1.15 extracted from

  • Namboodiri, M.A.A.; Corigliano-Murphy, A.; Jiang, G.; Rollag, M.; Provencio, I.
    Murine aspartoacylase: cloning, expression and comparison with the human enzyme (2000), Mol. Brain Res., 77, 285-289.
    View publication on PubMed
Search for more literature for 3.5.1.15

Application

Application Comment Organism
medicine diagnosis of Canavan disease, an autosomal recessive neurodegenerative disorder characterized by aspartoacylase deficiency, technique for prenatal diagnosis Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene identified with human cDNA, cloned into a thioredoxin fusion vector and overexpressed in bacteria Mus musculus
hASPA gene cloned and overexpressed in bacteria Homo sapiens
putative aspartoacylase identified, homologous to human ASPA Prochlorococcus marinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3 0.5 N-acyl-aspartic acid recombinant enzyme, purified from bacteria Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 recombinant mASPA fusion protein, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-L-aspartic acid + H2O Homo sapiens
-
 acetate + aspartic acid
-
 ?
N-acetyl-L-aspartic acid + H2O Bos taurus
-
 acetate + aspartic acid
-
 ?
N-acetyl-L-aspartic acid + H2O Mus musculus
-
 acetate + aspartic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 bovine
-
Homo sapiens
-
 human
-
Mus musculus Q8R3P0 mouse
-
Prochlorococcus marinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, overexpressed in bacteria Homo sapiens
recombinant enzyme, overexpressed in bacteria Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
brain
-
 Bos taurus
-
brain
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-aspartic acid + H2O
-
 Homo sapiens acetate + aspartic acid
-
 ?
N-acetyl-L-aspartic acid + H2O
-
 Bos taurus acetate + aspartic acid
-
 ?
N-acetyl-L-aspartic acid + H2O
-
 Mus musculus acetate + aspartic acid
-
 ?
N-acetylalanine + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + alanine
-
 ?
N-acetylarginine + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + arginine
-
 ?
N-acetylasparagine + H2O 10% of the activity towards N-acetylaspartate Homo sapiens acetate + aspartate + NH3 product is aspartate, not asparagine, indicating the enzyme catalyzes deacetylation as well as hydrolysis of the beta acid amide ?
N-acetylasparagine + H2O 10% of the activity towards N-acetylaspartate Mus musculus acetate + aspartate + NH3 product is aspartate, not asparagine, indicating the enzyme catalyzes deacetylation as well as hydrolysis of the beta acid amide ?
N-acetylcysteine + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + cysteine
-
 ?
N-acetylglutamate + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + glutamate
-
 ?
N-acetylglutamine + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + glutamine
-
 ?
N-acetylleucine + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + leucine
-
 ?
N-acetyllysine + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + lysine
-
 ?
N-acetylmethionine + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + methionine
-
 ?
N-acetylphenylalanine + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + phenylalanine
-
 ?
N-acetylproline + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + proline
-
 ?
N-acetyltyrosine + H2O poor substrate, 0.1% of the activity towards N-acetyl-aspartate Mus musculus acetate + tyrosine
-
 ?
N-acyl L-aspartic acid + H2O
-
 Homo sapiens acetate + L-aspartate
-
 ?
N-acyl L-aspartic acid + H2O
-
 Bos taurus acetate + L-aspartate
-
 ?
N-acyl L-aspartic acid + H2O
-
 Mus musculus acetate + L-aspartate
-
 ?

Synonyms

Synonyms Comment Organism
human ASPA
-
 Homo sapiens
murine aspartoacylase mASPA Mus musculus