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Literature summary for 3.5.1.15 extracted from
- Purification, characterization, and localization of aspartoacylase from bovine brain (1991), J. Neurochem., 56, 129-135.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | enhances activity | Bos taurus |  |
| NP-40 | enhances activity | Bos taurus | |
| Triton X-100 | increases activity of the enzyme | Bos taurus | |
| Tween-20 | enhances activity | Bos taurus | |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | diagnosis of Canavan disease, an autosomal recessive neurodegenerative disorder characterized by aspartoacylase deficiency, technique for prenatal diagnosis | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Glycyl-L-aspartate | - |
Bos taurus | |
| N-carbobenzoxy-D-aspartic acid | - |
Bos taurus | |
| N-tert-butoxycarbonyl-D-aspartic acid-beta-benzyl ester | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.85 | - |
N-acyl-L-aspartic acid | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates activity 20-40% | Bos taurus | |
| Mg2+ | stimulates activity 20-40% | Bos taurus | |
| Mn2+ | stimulates activity 20-40%, above 0.1 mM inhibition | Bos taurus | |
| Zn2+ | stimulates activity 20-40%, above 0.1 mM inhibition | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-L-aspartic acid + H2O | Homo sapiens | - |
acetate + aspartic acid | - |
? | |
| N-acetyl-L-aspartic acid + H2O | Bos taurus | - |
acetate + aspartic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
bovine | - |
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| brain | - |
Bos taurus | - |
| skin fibroblast | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.019 | 0.02 | - |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-L-aspartic acid + H2O | - |
Homo sapiens | acetate + aspartic acid | - |
? | |
| N-acetyl-L-aspartic acid + H2O | - |
Bos taurus | acetate + aspartic acid | - |
? | |
| N-acyl L-aspartic acid + H2O | - |
Homo sapiens | acetate + L-aspartate | - |
? | |
| N-acyl L-aspartic acid + H2O | - |
Bos taurus | acetate + L-aspartate | - |
? | |
| N-carbobenzoxy-L-aspartate + H2O | - |
Bos taurus | ? | - |
? | |
| N-tert-butoxycarbonyl-L-aspartic acid alpha-benzyl ester + H2O | - |
Bos taurus | ? | - |
? | |
| N-tert-butoxycarbonyl-L-aspartic acid beta-benzyl ester + H2O | - |
Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE, reducing conditions | Bos taurus |
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