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Literature summary for 3.5.1.15 extracted from

  • D'Adamo, A.F.; Peisach, J.; Manner, G.; Weiler, C.T.
    N-Acetyl-aspartate amidohydrolase: Purification and properties (1977), J. Neurochem., 28, 739-744.
    View publication on PubMed
Search for more literature for 3.5.1.15

General Stability

General Stability Organism
pure enzyme extremely unstable Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
aspartic acid 0.002 M, 20-25% inhibition Rattus norvegicus
glutamic acid 0.002 M, 20-25% inhibition Rattus norvegicus
N-ethylmaleimide
-
 Rattus norvegicus
p-hydroxymercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 N-acyl L-aspartic acid
-
 Sus scrofa
0.2
-
 N-acyl L-aspartic acid brain enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-L-aspartic acid + H2O Rattus norvegicus
-
 acetate + aspartic acid
-
 ?
N-acetyl-L-aspartic acid + H2O Sus scrofa
-
 acetate + aspartic acid
-
 ?
N-acetyl-L-aspartic acid + H2O Gallus sp.
-
 acetate + aspartic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus sp.
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
 pig
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
brain
-
 Sus scrofa
-
brain
-
 Gallus sp.
-
kidney
-
 Rattus norvegicus
-
kidney
-
 Sus scrofa
-
kidney
-
 Gallus sp.
-
liver
-
 Rattus norvegicus
-
liver
-
 Gallus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.47
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, frozen storage of undialyzed enzyme for a few days leads to a complete disappearance of activity following a single thaw Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information N-methyl aspartic acid, N-carbamoyl aspartic acid and N-glycyl aspartic acids are no substrates Rattus norvegicus ?
-
 ?
N-acetyl-L-aspartic acid + H2O
-
 Rattus norvegicus acetate + aspartic acid
-
 ?
N-acetyl-L-aspartic acid + H2O
-
 Sus scrofa acetate + aspartic acid
-
 ?
N-acetyl-L-aspartic acid + H2O
-
 Gallus sp. acetate + aspartic acid
-
 ?
N-acyl L-aspartic acid + H2O
-
 Rattus norvegicus acetate + L-aspartate
-
 ?
N-acyl L-aspartic acid + H2O
-
 Sus scrofa acetate + L-aspartate
-
 ?
N-acyl L-aspartic acid + H2O
-
 Gallus sp. acetate + L-aspartate
-
 ?
N-formyl aspartic acid + H2O
-
 Rattus norvegicus formate + aspartate
-
 ?

Synonyms

Synonyms Comment Organism
N-acetyl-aspartate deacetylase
-
 Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
 rapidly denatures above Rattus norvegicus
70
-
 stable at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
3 10
-
 Rattus norvegicus