Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol | Streptomyces sp. | |
PMSF | slight activation at 1 mM | Streptomyces sp. |
Cloned (Comment) | Organism |
---|---|
gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Streptomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
C141A | site-directed mutagenesis, inactive mutant | Streptomyces sp. |
E41A | site-directed mutagenesis, inactive mutant | Streptomyces sp. |
K107A | site-directed mutagenesis, inactive mutant | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Streptomyces sp. | |
Cd2+ | complete inhibition at 1 mM | Streptomyces sp. | |
Cu2+ | complete inhibition at 1 mM | Streptomyces sp. | |
Hg2+ | complete inhibition at 1 mM | Streptomyces sp. | |
additional information | no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for metal ions | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptomyces sp. | 4-guanidinobutyramide probably is the natural substrate | ? | - |
? | |
additional information | Streptomyces sp. 211726 | 4-guanidinobutyramide probably is the natural substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | A0A088BHP3 | gene azl13 | - |
Streptomyces sp. 211726 | A0A088BHP3 | gene azl13 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | halotolerant organism from mangrove swamps | Streptomyces sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.55 | - |
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide | Streptomyces sp. |
0.69 | - |
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide | Streptomyces sp. |
0.87 | - |
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate p-acetotoluidide | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',6'-dimethylacetanilide + H2O | - |
Streptomyces sp. | ? | - |
? | |
2',6'-dimethylacetanilide + H2O | - |
Streptomyces sp. 211726 | ? | - |
? | |
3',4'-dichloropropionanilide + H2O | i.e. propanil | Streptomyces sp. | 3,4-dichloroaniline + propionate | - |
? | |
3',4'-dichloropropionanilide + H2O | i.e. propanil | Streptomyces sp. 211726 | 3,4-dichloroaniline + propionate | - |
? | |
additional information | 4-guanidinobutyramide probably is the natural substrate | Streptomyces sp. | ? | - |
? | |
additional information | substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine | Streptomyces sp. | ? | - |
? | |
additional information | 4-guanidinobutyramide probably is the natural substrate | Streptomyces sp. 211726 | ? | - |
? | |
additional information | substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine | Streptomyces sp. 211726 | ? | - |
? | |
p-acetotoluidide + H2O | - |
Streptomyces sp. | ? | - |
? | |
p-acetotoluidide + H2O | - |
Streptomyces sp. 211726 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
aryl acylamidase | - |
Streptomyces sp. |
Azl13 | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Streptomyces sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 60 | activity range | Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified His6-tagged enzyme, pH 8.0, stable at for 1 h | Streptomyces sp. |
45 | 50 | purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining | Streptomyces sp. |
60 | - |
purified His6-tagged enzyme, pH 8.0, 1 h, inactivation | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | activity range | Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | purified His6-tagged enzyme, over 50% activity remaining within this range after 1 h | Streptomyces sp. |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the nitrilase superfamily, subfamily in branch 13. The enzyme contains highly conserved E41-K107-C141 catalytic triad which is typical for nitrilase superfamily amidases | Streptomyces sp. |