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Literature summary for 3.5.1.13 extracted from

  • Ma, Y.; Xu, W.; Zhang, J.; Zhang, S.; Hong, K.; Deng, Z.; Sun, Y.
    Nitrilase superfamily aryl acylamidase from the halotolerant mangrove Streptomyces sp. 211726 (2014), Appl. Microbiol. Biotechnol., 98, 8583-8590.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol Streptomyces sp.
PMSF slight activation at 1 mM Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
C141A site-directed mutagenesis, inactive mutant Streptomyces sp.
E41A site-directed mutagenesis, inactive mutant Streptomyces sp.
K107A site-directed mutagenesis, inactive mutant Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Streptomyces sp.
Cd2+ complete inhibition at 1 mM Streptomyces sp.
Cu2+ complete inhibition at 1 mM Streptomyces sp.
Hg2+ complete inhibition at 1 mM Streptomyces sp.
additional information no effect by EDTA, 8-hydroxyquinoline, and 2-mercaptoethanol Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for metal ions Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000, recombinant His-tagged enzyme, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptomyces sp. 4-guanidinobutyramide probably is the natural substrate ?
-
?
additional information Streptomyces sp. 211726 4-guanidinobutyramide probably is the natural substrate ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. A0A088BHP3 gene azl13
-
Streptomyces sp. 211726 A0A088BHP3 gene azl13
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information halotolerant organism from mangrove swamps Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide Streptomyces sp.
0.69
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide Streptomyces sp.
0.87
-
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate p-acetotoluidide Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',6'-dimethylacetanilide + H2O
-
Streptomyces sp. ?
-
?
2',6'-dimethylacetanilide + H2O
-
Streptomyces sp. 211726 ?
-
?
3',4'-dichloropropionanilide + H2O i.e. propanil Streptomyces sp. 3,4-dichloroaniline + propionate
-
?
3',4'-dichloropropionanilide + H2O i.e. propanil Streptomyces sp. 211726 3,4-dichloroaniline + propionate
-
?
additional information 4-guanidinobutyramide probably is the natural substrate Streptomyces sp. ?
-
?
additional information substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine Streptomyces sp. ?
-
?
additional information 4-guanidinobutyramide probably is the natural substrate Streptomyces sp. 211726 ?
-
?
additional information substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows amidase, EC 3.5.1.4, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with benzonitrile. phenylacetonitrile, Flutolanil, L-glutamine, and L-asparagine Streptomyces sp. 211726 ?
-
?
p-acetotoluidide + H2O
-
Streptomyces sp. ?
-
?
p-acetotoluidide + H2O
-
Streptomyces sp. 211726 ?
-
?

Subunits

Subunits Comment Organism
? x * 31000, recombinant His-tagged enzyme, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
aryl acylamidase
-
Streptomyces sp.
Azl13
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 60 activity range Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified His6-tagged enzyme, pH 8.0, stable at for 1 h Streptomyces sp.
45 50 purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining Streptomyces sp.
60
-
purified His6-tagged enzyme, pH 8.0, 1 h, inactivation Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 activity range Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10 purified His6-tagged enzyme, over 50% activity remaining within this range after 1 h Streptomyces sp.

General Information

General Information Comment Organism
evolution the enzyme belongs to the nitrilase superfamily, subfamily in branch 13. The enzyme contains highly conserved E41-K107-C141 catalytic triad which is typical for nitrilase superfamily amidases Streptomyces sp.