Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of DJ-1 knockdown in Drosophila cells in culture, and DJ-1beta knock-out flies. DJ-1beta knockdown does not affect the response of S2 cells to methylglyoxal challenge | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
S2 cell | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | neither His-tagged hDJ-1, nor untagged hDJ-1 show any deglycase activity in vitro, cysteine deglycase activity of enzyme DJ-1 is due to a buffer artifact, which can be attributed to TRIS buffer | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deglycase | - |
Drosophila melanogaster |
DJ-1 | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
malfunction | methylglyoxal causes formation of advanced glycation end-products on proteins, one of which is Nepsilon-carboxymethyllysine (CEL), which can be detected by immunoblotting whole cell lysates. Control S2 cells show little to no increase in CEL adducts when treated with 1 mM methylglyoxal. They are capable of efficiently detoxifying methylglyoxal. Knockdown of glyoxalase Glo1 causes a detectable impairment of methylglyoxal detoxification activity in vivo, since CEL adducts are clearly increased upon treatment with 1 mM methylglyoxal. In contrast, DJ-1 knockdown causes no detectable increase in CEL adducts compared with control cells, and combined knockdown of DJ-1beta and Glo1 shows no additional phenotype compared with Glo1 knockdown alone | Drosophila melanogaster |
physiological function | using both DJ-1 knockdown in Drosophila cells in culture, and DJ-1beta knock-out flies, no contribution of DJ-1 to survival to methylglyoxal challenge or to accumulation of methylglyoxal protein adducts can be detected | Drosophila melanogaster |