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Literature summary for 3.5.1.124 extracted from

  • Pfaff, D.H.; Fleming, T.; Nawroth, P.; Teleman, A.A.
    Evidence against a role for the Parkinsonism-associated protein DJ-1 in methylglyoxal detoxification (2017), J. Biol. Chem., 292, 685-690.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information construction of DJ-1 knockdown in Drosophila cells in culture, and DJ-1beta knock-out flies. DJ-1beta knockdown does not affect the response of S2 cells to methylglyoxal challenge Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
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-
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Source Tissue

Source Tissue Comment Organism Textmining
S2 cell
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 Drosophila melanogaster
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information neither His-tagged hDJ-1, nor untagged hDJ-1 show any deglycase activity in vitro, cysteine deglycase activity of enzyme DJ-1 is due to a buffer artifact, which can be attributed to TRIS buffer Drosophila melanogaster ?
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 ?

Synonyms

Synonyms Comment Organism
deglycase
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 Drosophila melanogaster
DJ-1
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 Drosophila melanogaster

General Information

General Information Comment Organism
malfunction methylglyoxal causes formation of advanced glycation end-products on proteins, one of which is Nepsilon-carboxymethyllysine (CEL), which can be detected by immunoblotting whole cell lysates. Control S2 cells show little to no increase in CEL adducts when treated with 1 mM methylglyoxal. They are capable of efficiently detoxifying methylglyoxal. Knockdown of glyoxalase Glo1 causes a detectable impairment of methylglyoxal detoxification activity in vivo, since CEL adducts are clearly increased upon treatment with 1 mM methylglyoxal. In contrast, DJ-1 knockdown causes no detectable increase in CEL adducts compared with control cells, and combined knockdown of DJ-1beta and Glo1 shows no additional phenotype compared with Glo1 knockdown alone Drosophila melanogaster
physiological function using both DJ-1 knockdown in Drosophila cells in culture, and DJ-1beta knock-out flies, no contribution of DJ-1 to survival to methylglyoxal challenge or to accumulation of methylglyoxal protein adducts can be detected Drosophila melanogaster