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Literature summary for 3.5.1.123 extracted from

  • Takeo, M.; Ohara, A.; Sakae, S.; Okamoto, Y.; Kitamura, C.; Kato, D.; Negoro, S.
    Function of a glutamine synthetase-like protein in bacterial aniline oxidation via gamma-glutamylanilide (2013), J. Bacteriol., 195, 4406-4414.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.123

Cloned(Commentary)

Cloned (Comment) Organism
gene atdA2, recombinant expression in enzyme-deficient, non-catechol-degrading Pseudomonas putida strain KT2440 Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Acinetobacter sp. a recombinant Pseudomonas strain harboring only atdA2 hydrolyzes gamma-glutamate into aniline, reversing the gamma-glutamate formation by AtdA1, a glutamine synthetase (GS)-like protein, EC 6.3.1.18 ?
-
 ?
N5-phenyl-L-glutamine + H2O Acinetobacter sp.
-
 L-glutamate + aniline
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
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-
-
Acinetobacter sp. YAA
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a recombinant Pseudomonas strain harboring only atdA2 hydrolyzes gamma-glutamate into aniline, reversing the gamma-glutamate formation by AtdA1, a glutamine synthetase (GS)-like protein, EC 6.3.1.18 Acinetobacter sp. ?
-
 ?
N5-phenyl-L-glutamine + H2O
-
 Acinetobacter sp. L-glutamate + aniline
-
 ?

Synonyms

Synonyms Comment Organism
atdA2
-
 Acinetobacter sp.
glutamine amidotransferase-like protein
-
 Acinetobacter sp.

General Information

General Information Comment Organism
metabolism the enzyme, encoded by gene atdA2, is involved in aniline oxidation as a part of the aniline degradation gene cluster, catechol is the major oxidation product from aniline, gamma-glutamate is an intermediate to catechol and a direct substrate for the dioxygenase. Acinetobacter sp. strain YAA has an aniline degradation gene cluster on the plasmid pYA1, the cluster consists of 14 genes (atdA1 to atdA5 and atdRSBCDEFGH) required for the conversion of aniline into tricarboxylic acid cycle intermediates. Putative aniline oxidation mechanism of Acinetobacter sp. YAA, pathway overview Acinetobacter sp.
physiological function enzyme AtdA2 prevents gamma-glutamate accumulation that is harmful to the cell Acinetobacter sp.