Any feedback?
Literature summary for 3.5.1.12 extracted from
- Biotinidase (1990), Methods Enzymol., 184, 103-111.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | plasma biotinidase activity assay for diagnosis and screen newborn infants for biotinidase deficiency, treatment of inborn metabolism errors with large doses of biotin | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Enterococcus faecalis |  |
| biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Homo sapiens | |
| biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Lacticaseibacillus casei | |
| biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Sus scrofa | |
| CuCl2 | - |
Homo sapiens | |
| CuCl2 | - |
Rattus rattus | |
| diisopropylfluorophosphate | - |
Lacticaseibacillus casei | |
| diisopropylfluorophosphate | - |
Rattus rattus | |
| diisopropylfluorophosphate | - |
Sus scrofa | |
| monoiodoacetate | - |
Homo sapiens | |
| monoiodoacetate | - |
Sus scrofa | |
| p-hydroxymercuribenzoate | not inhibited | Enterococcus faecalis | |
| p-hydroxymercuribenzoate | - |
Homo sapiens | |
| p-hydroxymercuribenzoate | not inhibited | Lacticaseibacillus casei | |
| p-hydroxymercuribenzoate | - |
Rattus rattus | |
| p-hydroxymercuribenzoate | - |
Sus scrofa | |
| phenylmethylsulfonyl fluoride | - |
Homo sapiens | |
| ZnCl2 | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
epsilon-N-biotinyl-L-lysine | - |
Homo sapiens | |
| 0.0062 | - |
epsilon-N-biotinyl-L-lysine | human plasma | Homo sapiens | |
| 0.0077 | - |
epsilon-N-biotinyl-L-lysine | - |
Homo sapiens | |
| 0.0078 | - |
epsilon-N-biotinyl-L-lysine | - |
Homo sapiens | |
| 0.008 | - |
epsilon-N-biotinyl-L-lysine | serum | Sus scrofa | |
| 0.0306 | - |
p-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
| 0.0319 | - |
p-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
| 0.0333 | - |
p-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
| 0.0341 | - |
p-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
| 0.055 | - |
biotinyl-4-aminobenzoic acid | porcine serum | Sus scrofa | |
| 0.1002 | - |
m-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | rough endoplasmatic reticulum and Golgi apparatus | Rattus rattus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | 76000 | SDS-PAGE, sedimentation analysis | Homo sapiens |
| 76000 | - |
- |
Sus scrofa |
| 76000 | - |
gel filtration | Homo sapiens |
| 110000 | - |
- |
Homo sapiens |
| 115000 | - |
- |
Homo sapiens |
| 115000 | - |
gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biotin amide + H2O | Enterococcus faecalis | - |
? | - |
? | |
| biotin amide + H2O | Lacticaseibacillus casei | - |
? | - |
? | |
| biotin amide + H2O | Rattus rattus | - |
? | - |
? | |
| biotin amide + H2O | Homo sapiens | cleaves D-biotinylamides and esters, recycling of the vitamin | ? | - |
? | |
| biotin amide + H2O | Sus scrofa | cleaves D-biotinylamides and esters, recycling of the vitamin | ? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | Homo sapiens | - |
? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | Sus scrofa | - |
? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | Enterococcus faecalis | - |
? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | Lacticaseibacillus casei | - |
? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | Rattus rattus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | - |
- |
- |
| Homo sapiens | - |
human | - |
| Lacticaseibacillus casei | - |
- |
- |
| Rattus rattus | - |
rat | - |
| Sus scrofa | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterococcus faecalis |
- |
Lacticaseibacillus casei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Homo sapiens | - |
| blood | serum, plasma, leukocytes | Homo sapiens | - |
| blood | serum, plasma, leukocytes | Sus scrofa | - |
| blood | serum, plasma, leukocytes | Rattus rattus | - |
| fibroblast | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| kidney | - |
Sus scrofa | - |
| kidney | - |
Rattus rattus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Sus scrofa | - |
| liver | - |
Rattus rattus | - |
| small intestine | - |
Homo sapiens | - |
| small intestine | - |
Rattus rattus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C stable | Homo sapiens |
| 4°C, 0.1 M phosphate buffer pH 6.0, 1 mM 2-mercaptoethanol, 1 mM EDTA, 1 month without loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biotin amide + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| biotin amide + H2O | - |
Lacticaseibacillus casei | ? | - |
? | |
| biotin amide + H2O | - |
Rattus rattus | ? | - |
? | |
| biotin amide + H2O | cleaves D-biotinylamides and esters, recycling of the vitamin | Homo sapiens | ? | - |
? | |
| biotin amide + H2O | cleaves D-biotinylamides and esters, recycling of the vitamin | Sus scrofa | ? | - |
? | |
| biotinyl-4-aminobenzoic acid + H2O | - |
Homo sapiens | biotin + 4-aminobenzoic acid | - |
? | |
| biotinyl-4-aminobenzoic acid + H2O | - |
Sus scrofa | biotin + 4-aminobenzoic acid | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Sus scrofa | biotin + L-lysine | - |
ir | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Enterococcus faecalis | biotin + L-lysine | - |
ir | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Lacticaseibacillus casei | biotin + L-lysine | - |
ir | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Rattus rattus | biotin + L-lysine | - |
ir | |
| epsilon-N-biotinyl-L-lysine + H2O | cannot cleave biocytin that is bound to avidin | Homo sapiens | biotin + L-lysine | - |
ir | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | ? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Sus scrofa | ? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Lacticaseibacillus casei | ? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Rattus rattus | ? | - |
? | |
| N-(+)biotinyl-4-aminobenzoic acid + H2O | - |
Homo sapiens | 4-aminobenzoic acid + biotin | - |
? | |
| N-biotinyl-3-aminobenzoic acid + H2O | - |
Homo sapiens | biotin + 3-aminobenzoic acid | - |
? | |
| N-biotinyl-3-aminobenzoic acid + H2O | - |
Sus scrofa | biotin + 3-aminobenzoic acid | - |
? | |
| N-biotinyl-beta-alanine + H2O | - |
Sus scrofa | biotin + beta-alanine | - |
? | |
| N-biotinyl-beta-alanine + H2O | - |
Enterococcus faecalis | biotin + beta-alanine | - |
? | |
| N-biotinyl-beta-alanine + H2O | - |
Lacticaseibacillus casei | biotin + beta-alanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Homo sapiens |
| monomer | - |
Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | denaturation after 30 min at 60°C | Sus scrofa |
| 60 | 70 | 15 min 60°C 60% activity lost, 70°C activity completely lost | Homo sapiens |
| 60 | 70 | stable up to 60°C, stable 30 min at 60°C, at 70°C activity decreases after 10 min to 20-50% of the initial value | Rattus rattus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
