Application | Comment | Organism |
---|---|---|
medicine | since Dop is necessary for persistent infection by Mycobacterium tuberculosis, its selective inhibition holds potential for tuberculosis therapy | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | in order to facilitate high-throughput screens for Dop inhibitors, a time-resolved Förster resonance energy transfer-based assay for enzyme (Dop) function is deleveloped. The time-resolved Förster resonance energy transfer-based assay is successfully applied to determine the Michaelis constant for ATP-binding and to test the co-factor tolerance of the enzyme | Corynebacterium glutamicum | |
additional information | in order to facilitate high-throughput screens for Dop inhibitors, a time-resolved Förster resonance energy transfer-based assay for enzyme (Dop) function is deleveloped. The time-resolved Förster resonance energy transfer-based assay is successfully applied to determine the Michaelis constant for ATP-binding and to test the co-factor tolerance of the enzyme | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q8NQE1 | - |
- |
Corynebacterium glutamicum ATCC 13032 | Q8NQE1 | - |
- |
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
deamidase of Pup | - |
Mycobacterium tuberculosis |
deamidase of Pup | - |
Corynebacterium glutamicum |
Dop | - |
Mycobacterium tuberculosis |
Dop | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
37 | - |
assay at | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mycobacterium tuberculosis |
7.4 | - |
assay at | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | absolutely required for enzyme function | Mycobacterium tuberculosis | |
ATP | absolutely required for enzyme function | Corynebacterium glutamicum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is essential for the infectivity of Mycobacterium tuberculosisis | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | in order to facilitate high-throughput screens for Dop inhibitors, a time-resolved Förster resonance energy transfer-based assay for enzyme (Dop) function is deleveloped. The time-resolved Förster resonance energy transfer-based assay is successfully applied to determine the Michaelis constant for ATP-binding and to test the co-factor tolerance of the enzyme | Corynebacterium glutamicum | |
additional information | - |
additional information | in order to facilitate high-throughput screens for inhibitors, a time-resolved Förster resonance energy transfer-based assay for enzyme (Dop) function is deleveloped. The time-resolved Förster resonance energy transfer-based assay is successfully applied to determine the Michaelis constant for ATP-binding and to test the co-factor tolerance of the enzyme | Mycobacterium tuberculosis |