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Literature summary for 3.5.1.117 extracted from
- Enzymatic hydrolysis of nylons: quantification of the reaction rate of nylon hydrolase for thin-layered nylons (2014), Appl. Microbiol. Biotechnol., 98, 8751-8761.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene nylCp2, recombinant expression of enzyme quadruple mutant in Escherichia coli straiin JM109 | Agromyces sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D122G/H130Y/D36A/E263Q | site-directed mutagenesis, the mutant enzyme hydrolyzes polymeric nylon-6 powder, but the extent of the degradation is low | Agromyces sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Agromyces sp. | nylon hydrolase degrades various aliphatic nylons, including nylon-6 and nylon-66. Nylons are synthetic polymers made from (a) a dicarboxylic acid and a diamine (e.g., for nylon-66 production), (b) an amino acid that is able to undergo self-condensation, or (c) its lactam, such as epsilon-caprolactam (for nylon-6 production). The enzyme reaction proceeds by degrading the solid polymer to soluble oligomers (step 1), followed by degrading the released soluble oligomers into smaller oligomers and/or monomers (step 2). During the polymer degradations, these two steps should proceed simultaneously. Nylon hydrolase (NylC) attacks the polymer chains that are exposed to the solvent, especially at positions where hydrogen-bonding between the polymer chains is partially weakened | ? | - |
? |  |
| nylon-6 polymer + H2O | Agromyces sp. | nylon-6 is the preferred nyloln substrate | ? | - |
? | |
| nylon-66 polymer + H2O | Agromyces sp. | identification of the reaction product from the nylon-(66-co-64(0.32)) copolymer by nylon hydrolase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agromyces sp. | - |
gene nylCp2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant quadruple mutant from Escherichia colistraiin JM109 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Agromyces sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | nylon hydrolase degrades various aliphatic nylons, including nylon-6 and nylon-66. Nylons are synthetic polymers made from (a) a dicarboxylic acid and a diamine (e.g., for nylon-66 production), (b) an amino acid that is able to undergo self-condensation, or (c) its lactam, such as epsilon-caprolactam (for nylon-6 production). The enzyme reaction proceeds by degrading the solid polymer to soluble oligomers (step 1), followed by degrading the released soluble oligomers into smaller oligomers and/or monomers (step 2). During the polymer degradations, these two steps should proceed simultaneously. Nylon hydrolase (NylC) attacks the polymer chains that are exposed to the solvent, especially at positions where hydrogen-bonding between the polymer chains is partially weakened | Agromyces sp. | ? | - |
? | |
| additional information | development and evaluation of an assay method that enables a quantitative evaluation of the reaction rate of hydrolysis at the interface between the solid and aqueous phases and a quantitative comparison of the degradability for various polyamides, overview. Thin layer product identification | Agromyces sp. | ? | - |
? | |
| nylon-6 polymer + H2O | nylon-6 is the preferred nyloln substrate | Agromyces sp. | ? | - |
? | |
| nylon-6 polymer + H2O | usage of nylon powders as substrates, nylon-6 is the preferred nyloln substrate | Agromyces sp. | ? | - |
? | |
| nylon-66 polymer + H2O | identification of the reaction product from the nylon-(66-co-64(0.32)) copolymer by nylon hydrolase | Agromyces sp. | ? | - |
? | |
| nylon-66 polymer + H2O | usage of nylon powders as substratesm, nylon-66 is hydrolyzed by the hydrolase, although the extent of degradation of nylon-66 is approximately 60% of that for nylon-6. Identification of the reaction product from the nylon-(66-co-64(0.32)) copolymer by nylon hydrolase | Agromyces sp. | ? | - |
? | |
| [N-(6-aminohexanoyl)]n + H2O | linear Ahx-dimer, Ahx-trimer, and Ahx-tetramer, as well as Ahx-linear oligomer, and Ahx-cyclic dimer | Agromyces sp. | [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NylC | - |
Agromyces sp. |
| nylon hydrolase | - |
Agromyces sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Agromyces sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
assay at | Agromyces sp. |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzyme NylC is a member of the N-terminal nucleophile hydrolase superfamily | Agromyces sp. |
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