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Literature summary for 3.5.1.117 extracted from

  • Nagai, K.; Yasuhira, K.; Tanaka, Y.; Kato, D.; Takeo, M.; Higuchi, Y.; Negoro, S.; Shibata, N.
    Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp. KI72 (2013), Acta Crystallogr. Sect. F, 69, 1151-1154.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.117

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Arthrobacter sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, with ammonium sulfate as a precipitant in 0.1 M HEPES buffer pH 7.5 containing 0.2 M NaCl and 25% (v/v) glycerol Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9000
-
 1 * 27000 + 1 * 9000, SDS-PAGE Arthrobacter sp.
27000
-
 1 * 27000 + 1 * 9000, SDS-PAGE Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[N-(6-aminohexanoyl)]n + H2O Arthrobacter sp.
-
 [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x
-
 ?
[N-(6-aminohexanoyl)]n + H2O Arthrobacter sp. KI72
-
 [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. KI72
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q Sepharose column chromatography, Sephacryl S-200 and gel filtration Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[N-(6-aminohexanoyl)]n + H2O
-
 Arthrobacter sp. [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x
-
 ?
[N-(6-aminohexanoyl)]n + H2O
-
 Arthrobacter sp. KI72 [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 27000 + 1 * 9000, SDS-PAGE Arthrobacter sp.

Synonyms

Synonyms Comment Organism
NylC
-
 Arthrobacter sp.