Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.115 extracted from

  • Steffek, M.; Newton, G.L.; Av-Gay, Y.; Fahey, R.C.
    Characterization of Mycobacterium tuberculosis mycothiol S-conjugate amidase (2003), Biochemistry, 42, 12067-12076.
    View publication on PubMed
Search for more literature for 3.5.1.115

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.19% residual activity at 1 mM 1,10-phenanthroline Mycobacterium tuberculosis
1,7-phenanthroline slightly more than 50% inhibition at 1 mM 1,7-phenanthroline Mycobacterium tuberculosis
mycothiol about 20% residual activity at 2 mM mycothiol Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 RifS13 in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
0.44
-
 mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
0.45
-
 RifS17 in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
0.65
-
 mycothiol-cerulenin in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
0.69
-
 mycothiol-acetophenone in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
1.3
-
 mycothiol-N-ethylmaleimide in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
3
-
 mycothiol-3-(N-maleimidopropionyl)biocytin Km above 3.0 mM, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
4.8
-
 mycothiol disulfide in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the protein contains 2.6 equivalents of Ca2+ per subunit after purification Mycobacterium tuberculosis
Zn2+ the protein contains 1.4 equivalents of Zn2+ per subunit after purification Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32697
-
 x * 32697, calculated from amino acid sequence Mycobacterium tuberculosis
33000
-
 x * 33000, SDA-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a mycothiol S-conjugate + H2O Mycobacterium tuberculosis the enzyme is extremely specific for the mycothiol portion of the mycothiol S-conjugate and relatively nonspecific with respect to the R-moiety an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
-
 ?
a mycothiol S-conjugate + H2O Mycobacterium tuberculosis H37Rv the enzyme is extremely specific for the mycothiol portion of the mycothiol S-conjugate and relatively nonspecific with respect to the R-moiety an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Sephadex G-25 gel filtration Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
 crude extract, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
10.6
-
 after 82fold purification, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a mycothiol S-conjugate + H2O the enzyme is extremely specific for the mycothiol portion of the mycothiol S-conjugate and relatively nonspecific with respect to the R-moiety Mycobacterium tuberculosis an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
-
 ?
a mycothiol S-conjugate + H2O the enzyme is extremely specific for the mycothiol portion of the mycothiol S-conjugate and relatively nonspecific with respect to the R-moiety Mycobacterium tuberculosis H37Rv an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
-
 ?
additional information the enzyme is highly specific for the mycothiol moiety of mycothiol S-conjugates and relatively nonspecific for the structure of the sulfur-linked conjugate Mycobacterium tuberculosis ?
-
 ?
additional information the enzyme is highly specific for the mycothiol moiety of mycothiol S-conjugates and relatively nonspecific for the structure of the sulfur-linked conjugate Mycobacterium tuberculosis H37Rv ?
-
 ?
mycothiol disulfide + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
mycothiol disulfide + H2O
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
mycothiol-3-(N-maleimidopropionyl)biocytin + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
mycothiol-3-(N-maleimidopropionyl)biocytin + H2O
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin + H2O
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
mycothiol-acetophenone + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
mycothiol-cerulenin + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
mycothiol-N-ethylmaleimide + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
RifS13 + H2O
-
 Mycobacterium tuberculosis 1-D-myo-inositol-alpha-D-glucopyranoside + N-acetyl-S-[(2R,12Z,14E,16S,18S,19S,20S,21S,22S,23S,24E)-5,6,9,17,19-pentahydroxy-23-methoxy-21-(methoxycarbonyl)-2,4,12,16,18,20,22-heptamethyl-1,11-dioxo-1,2-dihydro-2,7-(epoxypentadeca[1,11,13]trienoimino)naphtho[2,1-b]furan-8-yl]-L-cysteine
-
 ?
RifS17 + H2O
-
 Mycobacterium tuberculosis 1-D-myo-inositol-alpha-D-glucopyranoside + N-acetyl-S-[(2R,12Z,14E,16S,18S,19S,20S,21S,22S,23S,24E)-5,17,19-trihydroxy-23-methoxy-21-(methoxycarbonyl)-2,4,12,16,18,20,22-heptamethyl-1,6,9,11-tetraoxo-1,2,6,9-tetrahydro-2,7-(epoxypentadeca[1,11,13]trienoimino)naphtho[2,1-b]furan-8-yl]-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
? x * 32697, calculated from amino acid sequence Mycobacterium tuberculosis
? x * 33000, SDA-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
MCA
-
 Mycobacterium tuberculosis
Rv1082
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.82
-
 RifS13 in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
1.04
-
 RifS17 in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
1.3
-
 mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
1.7
-
 mycothiol disulfide in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
3
-
 mycothiol-3-(N-maleimidopropionyl)biocytin kcat above 3.0 s-1, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
7
-
 mycothiol-cerulenin in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
15
-
 mycothiol-N-ethylmaleimide in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
37
-
 mycothiol-acetophenone in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.35
-
 mycothiol disulfide in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
1.03
-
 mycothiol-3-(N-maleimidopropionyl)biocytin in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
2.3
-
 RifS17 in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
3
-
 mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
4.3
-
 RifS13 in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
11
-
 mycothiol-cerulenin in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
12
-
 mycothiol-N-ethylmaleimide in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis
54
-
 mycothiol-acetophenone in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C Mycobacterium tuberculosis