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Literature summary for 3.5.1.114 extracted from

  • Tsirulnikov, K.; Abuladze, N.; Newman, D.; Ryazantsev, S.; Wolak, T.; Magilnick, N.; Koag, M.C.; Kurtz, I.; Pushkin, A.
    Mouse aminoacylase 3: a metalloenzyme activated by cobalt and nickel (2009), Biochim. Biophys. Acta, 1794, 1049-1057.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.114

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
D68A inactive mutant enzyme Mus musculus
E177A inactive mutant enzyme Mus musculus
E24A inactive mutant enzyme Mus musculus
H116A inactive mutant enzyme Mus musculus
H21A inactive mutant enzyme Mus musculus
R63A inactive mutant enzyme Mus musculus
Y287A inactive mutant enzyme Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme D114A Mus musculus
0.22
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme Y156A Mus musculus
0.23
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme Y287A Mus musculus
0.23
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme Y287A Mus musculus
0.25
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme E289A Mus musculus
0.25
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme D114A Mus musculus
0.25
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme E289A Mus musculus
0.26
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme K290A Mus musculus
0.26
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme R67D Mus musculus
0.27
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme K290A Mus musculus
0.53
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme R67D Mus musculus
0.55
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme D235R Mus musculus
0.56
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, wild-type enzyme Mus musculus
0.61
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme E167R Mus musculus
0.64
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme E167A Mus musculus
0.71
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme R71A Mus musculus
0.79
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme Y156A Mus musculus
0.83
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme E167A Mus musculus
1.11
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme K290A Mus musculus
1.3
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme E167R Mus musculus
1.3
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme D235R Mus musculus
1.3
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, wild-type enzyme Mus musculus
2.02
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme R71A Mus musculus
2.46
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme D114A Mus musculus
2.48
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme N70A Mus musculus
2.8
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme N70A Mus musculus
2.83
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme N70A Mus musculus
3.02
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, activated by Co2+, wild-type enzyme Mus musculus
3.4
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme E289A Mus musculus
3.49
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme N70A Mus musculus
3.71
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme Y288A Mus musculus
4.21
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme Y287A Mus musculus
4.51
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, without Co2+, wild-type enzyme Mus musculus
5.14
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme D114A Mus musculus
5.2
-
 N-acetyl-L-tyrosine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme Y288A Mus musculus
6.76
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme Y288A Mus musculus
7
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme R71A Mus musculus
11
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme K290A Mus musculus
11.03
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme E289A Mus musculus
12.4
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme R71A Mus musculus
13.61
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, without Co2+, mutant enzyme Y287A Mus musculus
17.73
-
 N-acetyl-1,2-dichlorovinyl-L-cysteine pH 7.5, temperature not specified in the publication, activated by Co2+, mutant enzyme Y288A Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Co2+ and to a lesser extent Ni2+ increases activity several times in comparison with intact wild type AA3. Co2+ drastically increases the rate of deacetylation of N-acetyl-1,2-dichlorovinyl-L-cysteine and significantly increased the toxicity of Ac-DCVC in the HEK293T cells expressing wt-AA3. Aminoacylase 3 is a metalloenzyme significantly activated by Co2+ and Ni2+. The Co2+ -activated enzyme contains about 1 cobalt atom per monomer and trace amounts of zinc and other metals indicating that cobalt completely replaces them from the enzyme Mus musculus
additional information Mn2+, Fe2+, Cd2+, Mg2+, Ca2+, K+ and Na+ do not significantly alter activity Mus musculus
Ni2+ Co2+ and to a lesser extent Ni2+ increases activity several times in comparison with intact wild type AA3. Co2+ drastically increases the rate of deacetylation of N-acetyl-1,2-dichlorovinyl-L-cysteine and significantly increased the toxicity of Ac-DCVC in the HEK293T cells expressing wt-AA3. Aminoacylase 3 is a metalloenzyme significantly activated by Co2+ and Ni2+ Mus musculus
Zn2+ Wild type mouse aminoacylase 3 expressed in Escherichia coli contains 0.35 zinc atoms per monomer Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q91XE4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-1,2-dichlorovinyl-L-cysteine + H2O
-
 Mus musculus acetate + 1,2-dichlorovinyl-L-cysteine
-
 ?
N-acetyl-L-tyrosine + H2O
-
 Mus musculus acetate + L-tyrosine
-
 ?

Synonyms

Synonyms Comment Organism
AA3
-
 Mus musculus