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Literature summary for 3.5.1.114 extracted from

  • Pushkin, A.; Carpenito, G.; Abuladze, N.; Newman, D.; Tsuprun, V.; Ryazantsev, S.; Motemoturu, S.; Sassani, P.; Solovieva, N.; Dukkipati, R.; Kurtz, I.
    Structural characterization, tissue distribution, and functional expression of murine aminoacylase III (2003), Am. J. Physiol. Cell Physiol., 286, C848-856.
    View publication on PubMed
Search for more literature for 3.5.1.114

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells Mus musculus
expression in HEK-293 cells Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 N-acetyl-S-benzyl-L-cysteine pH 7.5, 37°C Mus musculus
1.4
-
 N-acetyl-L-tyrosine pH 7.5, 37°C Mus musculus
1.6
-
 N-acetyl-L-phenylalanine pH 7.5, 37°C Mus musculus
1.8
-
 N-acetyl-L-histidine pH 7.5, 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm predominant cytoplasmic localization of aminoacylase III Mus musculus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 4 * 35000, the monomers of the enzyme are arranged with a fourfold rotational symmetry, SDS-PAGE Mus musculus
35188
-
 4 * 35188, calculated from sequence Mus musculus
140000
-
 gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q91XE4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme has consensus sites for N-glycosylation (Asn70 and Asn117), tyrosine sulfation (Tyr88, Tyr165, and Tyr272), protein kinase C (Thr298), and casein kinase II (Thr83, Ser130, Ser160, Thr201, and Ser266) phosphorylation, and myristylation (Gly18, Gly19, Gly22, Gly27, and Gly185) Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain less expression Mus musculus
-
heart less expression Mus musculus
-
kidney the enzyme is expressed predominantly in kidney, localized predominantly to the apical domain of S1 proximal tubules and the cytoplasm of S2 and S3 proximal tubules. The data suggest that in kidney proximal tubules, aminoacylase III plays an important role in deacetylating mercapturic acids Mus musculus
-
liver less expression Mus musculus
-
lung less expression Mus musculus
-
small intestine less expression Mus musculus
-
stomach less expression Mus musculus
-
testis less expression Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme uses as a substrate the N-acetylated L-aromatic amino acids tyrosine, phenylalanine, and tryptophan Mus musculus ?
-
 ?
N-acetyl-L-histidine + H2O
-
 Mus musculus acetate + L-histidine
-
 ?
N-acetyl-L-phenylalanine + H2O
-
 Mus musculus acetate + L-phenylalanine
-
 ?
N-acetyl-L-tyrosine + H2O
-
 Mus musculus acetate + L-tyrosine
-
 ?
N-acetyl-S-benzyl-L-cysteine + H2O
-
 Mus musculus acetate + S-benzyl-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 35000, the monomers of the enzyme are arranged with a fourfold rotational symmetry, SDS-PAGE Mus musculus
tetramer 4 * 35188, calculated from sequence Mus musculus

Synonyms

Synonyms Comment Organism
Acy3
-
 Mus musculus
aminoacylase III
-
 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 substrate: N-acetyl-S-benzyl-L-cysteine Mus musculus
7.6
-
 substrate: N-acetyl-L-histidine or N-acetyl-L-tyrosine Mus musculus
7.7
-
 substrate: N-acetyl-L-phenylalanine Mus musculus

General Information

General Information Comment Organism
physiological function aminoacylase III contributes to the nephrotoxicity to cysteine S-conjugates Mus musculus