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Literature summary for 3.5.1.109 extracted from

  • Higuchi, K.; Hara, J.; Okamoto, R.; Kawashima, M.; Imokawa, G.
    The skin of atopic dermatitis patients contains a novel enzyme, glucosylceramide sphingomyelin deacylase, which cleaves the N-acyl linkage of sphingomyelin and glucosylceramide (2000), Biochem. J., 350, 747-756.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.109

Application

Application Comment Organism
medicine glucosylceramide sphingomyelin deacylase, is expressed in the skin of patients with atopic dermatitis, which plays an important role in ceramide deficiency (including acylceramides) in the stratum corneum Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 1 * 40000, SDS-PAGE Homo sapiens
42000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 patients with atopic dermatitis
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
a D-glucosyl-N-acylsphingosine + H2O = a carboxylate + D-glucosyl-sphingosine (2) Homo sapiens
a
an N-acyl-sphingosylphosphorylcholine + H2O = a carboxylate + sphingosylphosphorylcholine (1) Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
epidermis of patients with atopic dermatitis Homo sapiens
-
stratum corneum of patients with atopic dermatitis Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no hydrolysis of N-palmitoylsphingosine Homo sapiens ?
-
 ?
N-palmitoyl-D-glucosyl-sphingosine + H2O
-
 Homo sapiens palmitic acid + D-glucosyl-sphingosine
-
 ?
N-palmitoyl-sphingosylphosphorylcholine + H2O
-
 Homo sapiens palmitic acid + sphingosylphosphorylcholine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
GcSM deacylase
-
 Homo sapiens
glucosylceramide sphingomyelin deacylase
-
 Homo sapiens
SM deacylase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
 assay at Homo sapiens
5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4 7 pH 4.0: about 35% of maximal activity, pH 7.0: about 70% of maximal activity Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing
-
 4.2

General Information

General Information Comment Organism
physiological function glucosylceramide sphingomyelin deacylase, is expressed in the skin of patients with atopic dermatitis, which plays an important role in ceramide deficiency (including acylceramides) in the stratum corneum Homo sapiens