Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bordetella bronchiseptica |
Crystallization (Comment) | Organism |
---|---|
to 2.4 A resolution. Enzyme belongs to the cysteine hydrolase superfamily. The structure shows a conserved catalytic triad, residues Asp29, Lys117, and Cys150, observed in the proximity of a conserved non-proline cis-peptide bond within a small cavity that is likely the active site | Bordetella bronchiseptica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.128 | - |
Maleamate | pH 7.5, 25°C | Bordetella bronchiseptica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | NicF structure and activity do not depend on Zn2+ | Bordetella bronchiseptica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44100 | - |
4 * 44100, calculated | Bordetella bronchiseptica |
86000 | - |
gel fitlration | Bordetella bronchiseptica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | A0A0H3LKK8 | - |
- |
Bordetella bronchiseptica RB50 | A0A0H3LKK8 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
maleamate + H2O = maleate + NH3 | hydrolysis of maleamate is proposed to proceed by a nucleophilic addition?elimination sequence involving the thiolate side chain of Cys150 | Bordetella bronchiseptica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maleamate + H2O | - |
Bordetella bronchiseptica | maleate + NH3 | - |
? | |
maleamate + H2O | - |
Bordetella bronchiseptica RB50 | maleate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 44100, calculated | Bordetella bronchiseptica |
Synonyms | Comment | Organism |
---|---|---|
Bb1774 | - |
Bordetella bronchiseptica |
NicF | - |
Bordetella bronchiseptica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.7 | - |
Maleamate | pH 7.5, 25°C | Bordetella bronchiseptica |