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Literature summary for 3.5.1.105 extracted from
- Biocatalytic production of glucosamine from N-acetylglucosamine by diacetylchitobiose deacetylase (2018), J. Microbiol. Biotechnol., 28, 1850-1858 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | biocatalytic production of glucosamine from N-acetylglucosamine by diacetylchitobiose deacetylase. Although both strains are biocatalytically active, the performance of Bacillus subtilis is 2fold more effective. Establishment of an efficient biotransformation process for the biotechnological production of GlcN in Bacillus subtilis that is more environmentally friendly than the traditional multistep chemical synthesis approach. The overall optimal conditions are 18.6 g/l cells at 40°C, pH 7.5 for 3 h in 3-l bioreactor | Pyrococcus horikoshii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PH0499, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) and in Bacillus subtilis strain WB600, subcloning in Escherichia coli strain JM109, method optimization, overview | Pyrococcus horikoshii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-D-glucosamine + H2O | Pyrococcus horikoshii | - |
D-glucosamine + acetate | - |
? |  |
| N-acetyl-D-glucosamine + H2O | Pyrococcus horikoshii DSM 12428 | - |
D-glucosamine + acetate | - |
? | |
| N-acetyl-D-glucosamine + H2O | Pyrococcus horikoshii NBRC 100139 | - |
D-glucosamine + acetate | - |
? | |
| N-acetyl-D-glucosamine + H2O | Pyrococcus horikoshii JCM 9974 | - |
D-glucosamine + acetate | - |
? | |
| N-acetyl-D-glucosamine + H2O | Pyrococcus horikoshii ATCC 700860 | - |
D-glucosamine + acetate | - |
? | |
| N-acetyl-D-glucosamine + H2O | Pyrococcus horikoshii OT-3 | - |
D-glucosamine + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | O58235 | - |
- |
| Pyrococcus horikoshii ATCC 700860 | O58235 | - |
- |
| Pyrococcus horikoshii DSM 12428 | O58235 | - |
- |
| Pyrococcus horikoshii JCM 9974 | O58235 | - |
- |
| Pyrococcus horikoshii NBRC 100139 | O58235 | - |
- |
| Pyrococcus horikoshii OT-3 | O58235 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-D-glucosamine + H2O | - |
Pyrococcus horikoshii | D-glucosamine + acetate | - |
? | |
| N-acetyl-D-glucosamine + H2O | - |
Pyrococcus horikoshii DSM 12428 | D-glucosamine + acetate | - |
? | |
| N-acetyl-D-glucosamine + H2O | - |
Pyrococcus horikoshii NBRC 100139 | D-glucosamine + acetate | - |
? | |
| N-acetyl-D-glucosamine + H2O | - |
Pyrococcus horikoshii JCM 9974 | D-glucosamine + acetate | - |
? | |
| N-acetyl-D-glucosamine + H2O | - |
Pyrococcus horikoshii ATCC 700860 | D-glucosamine + acetate | - |
? | |
| N-acetyl-D-glucosamine + H2O | - |
Pyrococcus horikoshii OT-3 | D-glucosamine + acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dacph | - |
Pyrococcus horikoshii |
| diacetylchitobiose deacetylase | - |
Pyrococcus horikoshii |
| PH0499 | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
recombinant enzyme | Pyrococcus horikoshii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | high activity at 40-90°C, 50% of maximal activity at 30°C | Pyrococcus horikoshii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
recombinant enzyme | Pyrococcus horikoshii |
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