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Literature summary for 3.5.1.105 extracted from

  • Kadokura, K.; Sakamoto, Y.; Saito, K.; Ikegami, T.; Hirano, T.; Hakamata, W.; Oku, T.; Nishio, T.
    Production of a recombinant chitin oligosaccharide deacetylase from Vibrio parahaemolyticus in the culture medium of Escherichia coli cells (2007), Biotechnol. Lett., 29, 1209-1215.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
production of a recombinant chitin oligosaccharide deacetylase from Vibrio parahaemolyticus in the culture medium of Escherichia coli cells. The concentration of the recombinant enzyme in the Escherichia coli culture medium is 150times higher than that of wild-type enzyme produced in the culture medium by Vibrio parahaemolyticus KN1699 Vibrio parahaemolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Vibrio parahaemolyticus

Organism

Organism UniProt Comment Textmining
Vibrio parahaemolyticus
-
KN1699
-
Vibrio parahaemolyticus KN1699
-
KN1699
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Vibrio parahaemolyticus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.2
-
-
Vibrio parahaemolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcNAc-beta-1,4-GlcNAc + H2O
-
Vibrio parahaemolyticus GlcNAc-beta-1,4-GlcN + acetate
-
?
GlcNAc-beta-1,4-GlcNAc + H2O
-
Vibrio parahaemolyticus KN1699 GlcNAc-beta-1,4-GlcN + acetate
-
?
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O 30% of the activity with GlcNAc-beta-1,4-GlcNAc Vibrio parahaemolyticus ?
-
?
GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O 30% of the activity with GlcNAc-beta-1,4-GlcNAc Vibrio parahaemolyticus KN1699 ?
-
?
additional information no activity with: GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc, GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc or GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc Vibrio parahaemolyticus ?
-
?
additional information no activity with: GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc, GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc or GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc Vibrio parahaemolyticus KN1699 ?
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Vibrio parahaemolyticus

Synonyms

Synonyms Comment Organism
chitin oligosaccharide deacetylase
-
Vibrio parahaemolyticus
Pa-COD
-
Vibrio parahaemolyticus