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Literature summary for 3.5.1.104 extracted from

  • Yang, S.; Zhang, F.; Kang, J.; Zhang, W.; Deng, G.; Xin, Y.; Ma, Y.
    Mycobacterium tuberculosis Rv1096 protein: gene cloning, protein expression, and peptidoglycan deacetylase activity (2014), BMC Microbiol., 14, 174.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.104

Cloned(Commentary)

Cloned (Comment) Organism
gene Rv1096, sequence comparisons, recombinant expression in Escherichia coli strain ER2566 and in Mycobacterium smegmatis strain mc2155, deacetylation of cell wall peptidoglycan by the recombinant enzyme leads to lysozyme resistance in Mycobacterium smegmatis Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.91
-
 peptidoglycan-N-acetyl-D-glucosamine recombinant enzyme, pH 7.0, 37°C Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Mycobacterium tuberculosis 5618
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates, best metal ion Mycobacterium tuberculosis
additional information metallo-dependent enzyme Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidoglycan-N-acetyl-D-glucosamine + H2O Mycobacterium tuberculosis
-
 peptidoglycan-D-glucosamine + acetate
-
 ?
peptidoglycan-N-acetyl-D-glucosamine + H2O Mycobacterium tuberculosis H37Rv
-
 peptidoglycan-D-glucosamine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis O53444 gene Rv1096
-
Mycobacterium tuberculosis H37Rv O53444 gene Rv1096
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidoglycan-N-acetyl-D-glucosamine + H2O
-
 Mycobacterium tuberculosis peptidoglycan-D-glucosamine + acetate
-
 ?
peptidoglycan-N-acetyl-D-glucosamine + H2O
-
 Mycobacterium tuberculosis H37Rv peptidoglycan-D-glucosamine + acetate
-
 ?

Synonyms

Synonyms Comment Organism
Rv1096 protein
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.099
-
 peptidoglycan-N-acetyl-D-glucosamine recombinant enzyme, pH 7.0, 37°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function many bacteria modulate and evade the immune defenses of their hosts through peptidoglycan deacetylation. Deacetylation of cell wall peptidoglycan by the recombinant enzyme leads to lysozyme resistance in Mycobacterium smegmatis Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.088
-
 peptidoglycan-N-acetyl-D-glucosamine recombinant enzyme, pH 7.0, 37°C Mycobacterium tuberculosis