Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.104 extracted from

  • Austin, C.M.; Maier, R.J.
    Aconitase-mediated posttranscriptional regulation of Helicobacter pylori peptidoglycan deacetylase (2013), J. Bacteriol., 195, 5316-5322.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.104

Cloned(Commentary)

Cloned (Comment) Organism
gene pgdA, in the wild type, pgdAmRNAhalf-life is 13 min, whereas the half-life for the acnB strain is 7 min, apo-AcnB binds to the 3'-untranslated region of the pgdA RNA transcript. AcnB-pgdA transcript binding is abolished by the addition of iron. Real-time quantitative PCR enzyme expression analysis and RNA footprinting Helicobacter pylori

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Helicobacter pylori 5618
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ abolishes the acnB-pgdA transcript binding leading to destabilization of the enzyme's mRNA Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidoglycan-N-acetyl-D-glucosamine + H2O Helicobacter pylori peptidoglycan consists of alternating N-acetylglucosamine and N-acetylmuramic acid residues connected by beta-1,4 bonds and cross-linked via short peptide bridges peptidoglycan-D-glucosamine + acetate
-
 ?
peptidoglycan-N-acetyl-D-glucosamine + H2O Helicobacter pylori ATCC 700392 peptidoglycan consists of alternating N-acetylglucosamine and N-acetylmuramic acid residues connected by beta-1,4 bonds and cross-linked via short peptide bridges peptidoglycan-D-glucosamine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori O25080
-
-
Helicobacter pylori ATCC 700392 O25080
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidoglycan-N-acetyl-D-glucosamine + H2O
-
 Helicobacter pylori peptidoglycan-D-glucosamine + acetate
-
 ?
peptidoglycan-N-acetyl-D-glucosamine + H2O peptidoglycan consists of alternating N-acetylglucosamine and N-acetylmuramic acid residues connected by beta-1,4 bonds and cross-linked via short peptide bridges Helicobacter pylori peptidoglycan-D-glucosamine + acetate
-
 ?
peptidoglycan-N-acetyl-D-glucosamine + H2O
-
 Helicobacter pylori ATCC 700392 peptidoglycan-D-glucosamine + acetate
-
 ?
peptidoglycan-N-acetyl-D-glucosamine + H2O peptidoglycan consists of alternating N-acetylglucosamine and N-acetylmuramic acid residues connected by beta-1,4 bonds and cross-linked via short peptide bridges Helicobacter pylori ATCC 700392 peptidoglycan-D-glucosamine + acetate
-
 ?

Synonyms

Synonyms Comment Organism
peptidoglycan deacetylase
-
 Helicobacter pylori
pgdA
-
 Helicobacter pylori

Expression

Organism Comment Expression
Helicobacter pylori PgdA is highly expressed under oxidative stress up

General Information

General Information Comment Organism
physiological function Helicobacter pylori is equipped with a peptidoglycan deacetylase (PgdA) that confers both pure peptidoglycan and whole bacterial resistance to lysozyme degradation, which hydrolyzes the beta-1,4 bonds in peptidoglycan. Under oxidative stress, PgdA is highly expressed and confers resistance to lysozyme in wild-type cells. Role of aconitase (AcnB) in Helicobacter pylori as a posttranscriptional regulator of the cell wall-modifying enzyme peptidoglycan deacetylase PgdA, apo-AcnB directly interacts with the pgdA transcript to enhance stability and increase deacetylase enzyme expression, which impacts in vivo survival Helicobacter pylori