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Literature summary for 3.5.1.10 extracted from

  • Krupenko, S.A.; Vlasov, A.P.; Wagner, C.
    On the role of conserved histidine 106 in 10-formyltetrahydrofolate dehydrogenase catalysis (2001), J. Biol. Chem., 276, 24030-24037.
    View publication on PubMed
Search for more literature for 3.5.1.10

Protein Variants

Protein Variants Comment Organism
H100A soluble, shows enzymic activity Rattus norvegicus
H106A insoluble, no enzymatic activity Rattus norvegicus
H106E insoluble, no enzymatic activity Rattus norvegicus
H106K insoluble, no enzymatic activity Rattus norvegicus
H106N insoluble, no enzymatic activity Rattus norvegicus
H106Q insoluble, no enzymatic activity Rattus norvegicus
H106R insoluble, no enzymatic activity Rattus norvegicus
H113A insoluble, shows enzymic activity Rattus norvegicus
H125A soluble, shows enzymic activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 10-formyltetrahydrofolate 30°C, pH 7.8, wild type Rattus norvegicus
0.0083
-
 10-formyltetrahydrofolate 30°C, pH 7.8, H125A mutant Rattus norvegicus
0.009
-
 10-formyltetrahydrofolate 30°C, pH 7.8, H113A mutant Rattus norvegicus
0.0095
-
 10-formyltetrahydrofolate 30°C, pH 7.8, H100A mutant Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate dehydrogenase reaction requires full length enzyme, hydrolase reaction occurs in amino-terminal domain of enzyme Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + H2O
-
 Rattus norvegicus formate + tetrahydrofolate
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADP+ required for dehydrogenase reaction Rattus norvegicus