Literature summary for 3.5.1.1 extracted from
Gladilina, Y.; Sokolov, N.; Krasotkina, J.
Cloning, expression, and purification of Helicobacter pylori L-asparaginase (2009), Biochemistry (Moscow) Suppl. Ser. B, 3, 89-91.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
cloned and expressed in Escherichia coli BL21 (DE3) cells |
Helicobacter pylori J99 |
Organism
Organism |
UniProt |
Comment |
Textmining |
Helicobacter pylori J99 |
Q9ZLB9 |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant protein, purified 1.8fold: sonication for preparation of cell free extract, soluble fraction of cell free extract, pH 7.2 and 5.8, chromatography on SP-Sepharose, more than 60% yield |
Helicobacter pylori J99 |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
92 |
- |
purified recombinant protein, pH 7.0, 37°C |
Helicobacter pylori J99 |
Synonyms
Synonyms |
Comment |
Organism |
HPA |
Helicobacter pylori L-asparaginase |
Helicobacter pylori J99 |
L-asparaginase |
- |
Helicobacter pylori J99 |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
10 |
- |
HpA rapidly and irreversible loses activity at pH 10 and above, whereas lysis at pH 9.0-9.5 requires prolonged incubation |
Helicobacter pylori J99 |
pI Value
Organism |
Comment |
pI Value Maximum |
pI Value |
Helicobacter pylori J99 |
recombinant protein determined by amino acid sequence |
- |
8.1 |