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Literature summary for 3.5.1.1 extracted from

  • Gladilina, Y.; Sokolov, N.; Krasotkina, J.
    Cloning, expression, and purification of Helicobacter pylori L-asparaginase (2009), Biochemistry (Moscow) Suppl. Ser. B, 3, 89-91.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli BL21 (DE3) cells Helicobacter pylori J99

Organism

Organism UniProt Comment Textmining
Helicobacter pylori J99 Q9ZLB9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purified 1.8fold: sonication for preparation of cell free extract, soluble fraction of cell free extract, pH 7.2 and 5.8, chromatography on SP-Sepharose, more than 60% yield Helicobacter pylori J99

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
92
-
purified recombinant protein, pH 7.0, 37°C Helicobacter pylori J99

Synonyms

Synonyms Comment Organism
HPA Helicobacter pylori L-asparaginase Helicobacter pylori J99
L-asparaginase
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Helicobacter pylori J99

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
HpA rapidly and irreversible loses activity at pH 10 and above, whereas lysis at pH 9.0-9.5 requires prolonged incubation Helicobacter pylori J99

pI Value

Organism Comment pI Value Maximum pI Value
Helicobacter pylori J99 recombinant protein determined by amino acid sequence
-
8.1