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Literature summary for 3.5.1.1 extracted from
- Probing the antigenicity of E. coli L-asparaginase by mutational analysis (2006), Mol. Biotechnol., 33, 57-65.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mutant enzymes in Escherichia coli | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R195A/K196A/H197A | alanine-scanning mutagenesis for determination of amino acid residues critical for antigenicity, construction of four mutants, the mutants' antigenicity is greatly reduced | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | residues 195RKH197 are critical for enzyme antigenicity | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant mutant enzymes from Escherichia coli | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | residues 195RKH197 are critical for enzyme antigenicity | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | residues 195RKH197 are critical for enzyme antigenicity | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-asparaginase | - |
Escherichia coli |
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