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Literature summary for 3.5.1.1 extracted from

  • Tosa, T.; Sano, R.; Yamamoto, K.; Nakamura, M.; Chibata, I.
    L-Asparaginase from Proteus vulgaris. Purification, crystallization, and enzymic properties (1972), Biochemistry, 11, 217-222.
    View publication on PubMed
Search for more literature for 3.5.1.1

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Proteus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 L-asparagine
-
 Proteus vulgaris
0.43
-
 D-Asn
-
 Proteus vulgaris
5
-
 L-Gln
-
 Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Proteus vulgaris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
300
-
-
 Proteus vulgaris

Storage Stability

Storage Stability Organism
-20°C, stable for 1 month Proteus vulgaris
5°C, as suspension in 3.5 M ammonium sulfate, stable for 1 year Proteus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Asn + H2O 10% of the activity with L-Asn Proteus vulgaris D-Asp + NH3
-
 ?
D-aspartic acid beta-hydroxamate + H2O 59% of the activity with L-Asn Proteus vulgaris D-Asp + hydroxylamine
-
 ?
L-Asn + H2O
-
 Proteus vulgaris L-Asp + NH3
-
 ?
L-aspartic acid beta-hydrazide + H2O 2% of the activity with L-Asn Proteus vulgaris L-Asp + hydroxylamine
-
 ?
L-aspartic acid beta-hydroxamate + H2O 59% of the activity with L-Asn Proteus vulgaris L-Asp + hydroxylamine
-
 ?
L-Gln + H2O 2% of the activity with L-Asn Proteus vulgaris L-Glu + NH3
-
 ?
L-glutamic acid gamma-hydroxamate + H2O 1% of the activity with L-Asn Proteus vulgaris L-Glu + hydroxylamine
-
 ?
Nalpha-methyl-L-Asn + H2O 26% of the activity with L-Asn Proteus vulgaris Nalpha-methyl-L-Asp + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
57
-
-
 Proteus vulgaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
32 62 32°C: about 60% of maximal activity, 62°C: about 85% pf maximal activity Proteus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Proteus vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
4 11 pH 4.0: about 50% of maximal activity, pH 11.0: about 75% of maximal activity Proteus vulgaris